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LMPD Database

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LMP007332

UniProt Annotations

Entry Information

Gene Name	bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
Protein Entry	DPP1_YEAST
UniProt ID	Q05521
Species	Yeast (S288c)

Comments

Comment type	Description
Biophysicochemical Properties	Kinetic parameters: Vmax=167 umol/min/mg enzyme with lysophosphatidic acid as substrate {ECO:0000269\|PubMed:8940025}; pH dependence: Optimum pH is 6.0-8.5. {ECO:0000269\|PubMed:8940025};
Catalytic Activity	1,2-diacyl-sn-glycerol 3-diphosphate + H(2)O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate. {ECO:0000269\|PubMed:8567632}.
Catalytic Activity	A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate. {ECO:0000269\|PubMed:8567632}.
Domain	The phosphatase sequence motif I (including Arg-125) and II (including His-169) are part of the cytoplasmic loop 2 and phosphatase sequence motif III (including His-223) is part of the cytoplasmic loop 3.
Enzyme Regulation	Inhibited by sodium fluoride (NaF) and pyrophosphate. Strongly inhibited by manganese ion and, to a lower extent, by magnesium and calcium ions. Also inhibited by Cu(2+) ion. In an indirect manner, it is also inhibited by the zinc ion which is able to form a complex with DGPP and prevent the enzyme from removing the phosphate from the substrate. Not inhibited by N-ethylmaleimide. {ECO:0000269\|PubMed:11139591, ECO:0000269\|PubMed:8567632}.
Function	Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Together with LPP1, regulates intracellular DGPP and PA levels, which are phospholipid molecules believed to play a signaling role in stress response. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. Substrate preference is DGPP > LPA > PA. Activity is independent of a divalent cation ion and insensitive to inhibition by N-ethylmaleimide. {ECO:0000269\|PubMed:11139591, ECO:0000269\|PubMed:8567632}.
Induction	Induced by the transcription factor ZAP1 during zinc depletion and negatively regulated by the transcription factor GIS1 predominantly during nutrient limitation. Induced by inositol supplementation. {ECO:0000269\|PubMed:11139591, ECO:0000269\|PubMed:12799368, ECO:0000269\|PubMed:14642771}.
Miscellaneous	Present with 3038 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the PA-phosphatase related phosphoesterase family. {ECO:0000305}.
Subcellular Location	Vacuole membrane {ECO:0000269\|PubMed:11139591, ECO:0000269\|PubMed:14562095, ECO:0000269\|PubMed:8567632}; Multi-pass membrane protein {ECO:0000269\|PubMed:11139591, ECO:0000269\|PubMed:14562095, ECO:0000269\|PubMed:8567632}.