LMPD Database

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LMP007087

UniProt Annotations

Entry Information
Gene Namepolyphosphatase DDP1
Protein EntryDDP1_YEAST
UniProt IDQ99321
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=31 nM for PP-InsP5 {ECO:0000269|PubMed:10419486};
Catalytic ActivityDiphospho-myo-inositol polyphosphate + H(2)O = myo-inositol polyphosphate + phosphate. {ECO:0000269|PubMed:10419486}.
Catalytic ActivityP(1),P(5)-bis(5'-adenosyl)pentaphosphate + H(2)O = adenosine 5'-tetraphosphate + AMP. {ECO:0000269|PubMed:10419486}.
Catalytic ActivityP(1),P(6)-bis(5'-adenosyl)hexaphosphate + H(2)O = adenosine 5'-pentaphosphate + AMP. {ECO:0000269|PubMed:10419486}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420;
FunctionMay eliminate potentially toxic dinucleoside polyphosphates during sporulation. Most active against diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A). Can also hydrolyze diadenosine 5',5'''-P1,P5-pentaphosphate (Ap5A), adenosine 5'-pentaphosphate, and adenosine 5'-tetraphosphate are also substrates, but not diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols. Also cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate).
Mass SpectrometryMass=21443; Method=Electrospray; Range=2-188; Evidence={ECO:0000269|PubMed:10085096};
MiscellaneousPresent with 3340 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
SimilarityBelongs to the Nudix hydrolase family. DIPP subfamily. {ECO:0000305}.
SimilarityContains 1 nudix hydrolase domain. {ECO:0000255|PROSITE-ProRule:PRU00794}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.