LMPD Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
LMP002768

UniProt Annotations

Entry Information
Gene Nameacyl-CoA thioesterase 2
Protein EntryACOT2_HUMAN
UniProt IDP49753
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P49753-1; Sequence=Displayed; Name=2; IsoId=P49753-2; Sequence=VSP_012225, VSP_012226;
Biophysicochemical PropertiesKinetic parameters: KM=40.3 uM for C10-acyl-CoA ; KM=8.9 uM for C12-acyl-CoA ; KM=1.6 uM for C14-acyl-CoA ; KM=2.0 uM for C16-acyl-CoA ; KM=2.8 uM for C18-acyl-CoA ; KM=4.8 uM for C20-acyl-CoA ; KM=4.5 uM for C16:1-acyl-CoA ; KM=6.1 uM for C18:1-acyl-CoA ; KM=4.3 uM for C18:1-trans-acyl-CoA ; Vmax=212 nmol/min/mg enzyme toward C10-acyl-CoA ; Vmax=681 nmol/min/mg enzyme toward C12-acyl-CoA ; Vmax=766 nmol/min/mg enzyme toward C14-acyl-CoA ; Vmax=656 nmol/min/mg enzyme toward C16-acyl-CoA ; Vmax=488 nmol/min/mg enzyme toward C18-acyl-CoA ; Vmax=408 nmol/min/mg enzyme toward C20-acyl-CoA ; Vmax=661 nmol/min/mg enzyme toward C16:1-acyl-CoA ; Vmax=304 nmol/min/mg enzyme toward C18:1-acyl-CoA ; Vmax=418 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA ;
Catalytic ActivityPalmitoyl-CoA + H(2)O = CoA + palmitate.
CautionWas originally (PubMed:10944470) thought to be peroxisomal but was later shown (PubMed:16940157) to be mitochondrial. {ECO
FunctionAcyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.
Sequence CautionSequence=AAC42007.1; Type=Frameshift; Positions=215, 226; Evidence= ;
SimilarityBelongs to the C/M/P thioester hydrolase family.
Subcellular LocationMitochondrion .
Tissue SpecificityStrongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas.