LMPD Database

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LMP000947

UniProt Annotations

Entry Information
Gene Namearachidonate lipoxygenase 3
Protein EntryLOXE3_HUMAN
UniProt IDQ9BYJ1
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9BYJ1-1; Sequence=Displayed; Name=2; IsoId=Q9BYJ1-2; Sequence=VSP_043287; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=46 uM for (12R)-HPETE {ECO
Catalytic ActivityA hydroperoxy icosatetraenoate = a hydroxy epoxy icosatrienoate.
Catalytic ActivityA hydroperoxy icosatetraenoate = an oxoicosatetraenoate + H(2)O.
CofactorName=Fe cation; Xref=ChEBI
DiseaseIchthyosis, congenital, autosomal recessive 3 (ARCI3) [MIM
FunctionNon-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl- sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia. {ECO
InteractionO75342:ALOX12B; NbExp=2; IntAct=EBI-6925949, EBI-6925925;
PathwayLipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
PathwayLipid metabolism; sphingolipid metabolism.
SimilarityBelongs to the lipoxygenase family.
SimilarityContains 1 lipoxygenase domain. {ECO
SimilarityContains 1 PLAT domain. {ECO
Subcellular LocationCytoplasm {ECO
Tissue SpecificityPredominantly expressed in skin.
Web ResourceName=Protein Spotlight; Note=about water - Issue 153 of September 2013; URL="http://web.expasy.org/spotlight/back_issues/153/";