LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP011407

UniProt Annotations

Entry Information
Gene Nametriacylglycerol lipase SDP1
Protein EntrySDP1_ARATH
UniProt IDQ9LZA6
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiespH dependence: Broad optimum pH around 8.0.;
Catalytic ActivityTriacylglycerol + H(2)O = diacylglycerol + a carboxylate. {ECO:0000269|PubMed:16473965}.
Developmental StageTranscript levels increase at the beginning of seed maturation and remain high until seed desiccation is completed. High levels early in seed imbibition, but decline as the seed germinates. {ECO:0000269|PubMed:16473965}.
Disruption PhenotypeNot impaired in germination, but much slower rate of postgerminative growth. {ECO:0000269|PubMed:16473965}.
Enzyme RegulationInhibited by diethyl-p-nitrophenyl phosphate, diisopropyl fluorophosphate and [E]-6-[bromoethylene]-3-[1- naphthalenyl]-2H-tetrahydropyran-2-one. {ECO:0000269|PubMed:16473965}.
FunctionInvolved it the release of fatty acids from the oil body in germinating seedlings. Could hydrolyze triacylglycerols and diacylglycerols but not monoacylglycerols, phospholipids, galactolipids or cholesterol esters. {ECO:0000269|PubMed:16473965, ECO:0000269|PubMed:19136267}.
MiscellaneousTranscript levels do not correlate positively with enzyme activity, suggesting a post-transcriptional regulation.
Sequence CautionSequence=BAD94338.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityContains 1 patatin domain. {ECO:0000305}.
Subcellular LocationLipid droplet {ECO:0000269|PubMed:16473965}. Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=Associates with the oil body membrane.