LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP010359

UniProt Annotations

Entry Information
Gene Namegeranylgeranyl transferase type-1 subunit beta
Protein EntryPGTB1_ARATH
UniProt IDO80642
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=5.8 uM for CVIM substrate {ECO:0000269|PubMed:11500541, ECO:0000269|PubMed:20565889}; KM=3.5 uM for CVIQ substrate {ECO:0000269|PubMed:11500541, ECO:0000269|PubMed:20565889}; KM=19.0 uM for CVII substrate {ECO:0000269|PubMed:11500541, ECO:0000269|PubMed:20565889}; KM=17.2 uM for CVIL substrate {ECO:0000269|PubMed:11500541, ECO:0000269|PubMed:20565889}; Note=Kcat is 0.5 h(-1), 0.08 h(-1), 0.008 h(-1) and 0.012 h(-1) for CVIL, CVII, CVIQ and CVIM substrates, respectively.; pH dependence: Optimum pH is 7.9-8.5 for CTIL substrate. {ECO:0000269|PubMed:11500541, ECO:0000269|PubMed:20565889}; Temperature dependence: Optimum temperature is 30-37 degrees Celsius for CTIL substrate. {ECO:0000269|PubMed:11500541, ECO:0000269|PubMed:20565889};
Catalytic ActivityGeranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
CofactorName=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Binds 1 zinc ion per subunit. {ECO:0000250};
Disruption PhenotypeNo visible phenotype under normal growth conditions, but mutant plants have an enhanced response to abscisic acid (ABA)-mediated stomatal closure and increased lateral root formation in response to exogenous auxin. {ECO:0000269|PubMed:16183844}.
FunctionCatalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X (CaaX). Seems to exclusively prenylate CaaX substrates with leucine in the terminal position. The beta subunit is responsible for peptide-binding. May negatively regulate abscisic acid (ABA) signaling in guard cells and auxin- induced lateral root initiation.
FunctionNegatively regulates ABA signaling in guard cells. in negative regulation of auxin-induced lateral root initiation.
Sequence CautionSequence=AAG40865.1; Type=Frameshift; Positions=106; Evidence={ECO:0000305};
SimilarityBelongs to the protein prenyltransferase subunit beta family. {ECO:0000305}.
SimilarityContains 4 PFTB repeats. {ECO:0000305}.
SubunitHeterodimer of an alpha and a beta subunit. {ECO:0000250}.
Tissue SpecificityExpressed in roots, leaves, stems, flowers and siliques. {ECO:0000269|PubMed:11500541}.