LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP010095

UniProt Annotations

Entry Information
Gene Namesulfotransferase 5
Protein EntrySOT5_ARATH
UniProt IDQ9M1V2
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=2 uM for kaempferol 3-sulfate {ECO:0000269|PubMed:17095238}; KM=6 uM for isorhamnetin {ECO:0000269|PubMed:17095238}; KM=3.2 uM for kaempferol {ECO:0000269|PubMed:17095238}; KM=11 uM for galangin {ECO:0000269|PubMed:17095238}; KM=1.5 uM for quercetin {ECO:0000269|PubMed:17095238}; KM=5.1 uM for apigenin 4'-sulfate {ECO:0000269|PubMed:17095238}; KM=51 uM for apigenin {ECO:0000269|PubMed:17095238}; KM=40.6 uM for chrysin {ECO:0000269|PubMed:17095238}; KM=26 uM for 7-hydroxyflavone {ECO:0000269|PubMed:17095238}; KM=1 uM for 3'-phospho-5'-adenylyl sulfate {ECO:0000269|PubMed:17095238}; Vmax=286 pmol/sec/mg enzyme with kaempferol 3-sulfate as substrate {ECO:0000269|PubMed:17095238}; Vmax=232 pmol/sec/mg enzyme with isorhamnetin as substrate {ECO:0000269|PubMed:17095238}; Vmax=116 pmol/sec/mg enzyme with kaempferol as substrate {ECO:0000269|PubMed:17095238}; Vmax=270 pmol/sec/mg enzyme with galangin as substrate {ECO:0000269|PubMed:17095238}; Vmax=24 pmol/sec/mg enzyme with quercetin as substrate {ECO:0000269|PubMed:17095238}; Vmax=45 pmol/sec/mg enzyme with apigenin 4'-sulfate as substrate {ECO:0000269|PubMed:17095238}; Vmax=370 pmol/sec/mg enzyme with apigenin as substrate {ECO:0000269|PubMed:17095238}; Vmax=156 pmol/sec/mg enzyme with chrysin as substrate {ECO:0000269|PubMed:17095238}; Vmax=91 pmol/sec/mg enzyme with 7-hydroxyflavone as substrate {ECO:0000269|PubMed:17095238}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:17095238};
Developmental StageExpressed mostly in 5-day old seedlings, at developmental stage 1. {ECO:0000269|PubMed:17095238}.
FunctionSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to specifically catalyze the sulfate conjugation of flavones and flavonols. Strictly specific for the position 7. Substrate preference is kaempferol 3-sulfate > isorhamnetin > kaempferol. {ECO:0000269|PubMed:17095238}.
InductionUp-regulated by trans-zeatin, but not by cold, heat, hypoxia, salt stress, auxins, gibberellins, wounding, etiolation and salicylic acid or methyl jasmonate treatments. {ECO:0000269|PubMed:17095238}.
SimilarityBelongs to the sulfotransferase 1 family. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000250}.
Tissue SpecificityExpressed in inflorescence stems, roots and siliques. {ECO:0000269|PubMed:17095238}.