LIPID MAPS® Gene/Proteome Database (LMPD)

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LMP010091

UniProt Annotations

Entry Information

Gene Name	sulphotransferase 12
Protein Entry	SOT12_ARATH
UniProt ID	P52839
Species	Arabidopsis

Comments

Comment type	Description
Biophysicochemical Properties	Kinetic parameters: KM=6.9 uM for 24-epicathasterone {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; KM=1.9 uM for 6-deoxo-24-epicathasterone {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; KM=3.0 uM for 17-beta-estradiol {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; KM=1.1 uM for dehydroepiandrosterone {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; KM=13 uM for pregnenolone {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; KM=0.44 mM for salicylic acid {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; KM=3 uM for 3'-phospho-5'-adenylyl sulfate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; Vmax=57 pmol/sec/mg enzyme with 24-epicathasterone as substrate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; Vmax=4.4 pmol/sec/mg enzyme with 24-epicathasterone as substrate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; Vmax=1.6 pmol/sec/mg enzyme with 17-beta-estradiol as substrate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; Vmax=1.3 pmol/sec/mg enzyme with dehydroepiandrosterone as substrate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; Vmax=5.9 pmol/sec/mg enzyme with pregnenolone as substrate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}; Vmax=2.67 pmol/sec/mg enzyme with salicylic acid as substrate {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532};
Disruption Phenotype	Hypersensitivity to NaCl and ABA in seed germination, and to salicylic acid (SA) in seedling growth. {ECO:0000269\|PubMed:20374532}.
Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the stereospecific sulfate conjugation of 24-epibrassinosteroids. Preferred substrates are 24-epicathasterone and 6-deoxo-24-epicathasterone. Low activity with 22-deoxy-24-epiteasterone. No activity with 24- epimers catasterone and brassinolide. Sulfonates salicylic acid. May be involved in detoxification. Enhances plant response to pathogen infection and contributes to long distance signaling in systemic acquired resistance (SAR). {ECO:0000269\|PubMed:17039368, ECO:0000269\|PubMed:20374532}.
Induction	Up-regulated by pathogens, methyljasmonate, salicylic acid, salt, osmotic stress, cold, auxin, cytokinin and abscisic acid treatments. Not induced by desiccation and ethylene treatment. {ECO:0000269\|PubMed:20374532, ECO:0000269\|PubMed:8639757, ECO:0000269\|PubMed:9212075}.
Sequence Caution	Sequence=CAA80546.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA86850.1; Type=Frameshift; Positions=300, 313, 317; Evidence={ECO:0000305};
Similarity	Belongs to the sulfotransferase 1 family. {ECO:0000305}.
Subcellular Location	Cytoplasm {ECO:0000250}.
Subunit	Dimer. {ECO:0000305\|PubMed:19173308}.
Tissue Specificity	Expressed in the aerial parts of seedlings, in roots, leaves and flowers. Not detected in stems and siliques. {ECO:0000269\|PubMed:20374532, ECO:0000269\|PubMed:8639757, ECO:0000269\|PubMed:9212075}.