LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP007446

UniProt Annotations

Entry Information
Gene NameLdh1p
Protein EntryLDH1_YEAST
UniProt IDP38139
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.77 uM for p-nitrophenyl butyrate (PNB) {ECO:0000269|PubMed:21478434}; KM=3.3 mM for 1,2-dioleoyl-3-(pyren-1-yl)-decanoyl-rac-glycerol (DPG) {ECO:0000269|PubMed:21478434}; Vmax=0.041 umol/min/mg enzyme for esterase activity using PNB as a substrate {ECO:0000269|PubMed:21478434}; Vmax=1 umol/min/mg enzyme for triacylglycerol lipase activity using DPG as a substrate {ECO:0000269|PubMed:21478434};
Biophysicochemical PropertiesKinetic parameters: KM=0.77 uM for p-nitrophenyl butyrate (PNB) ; KM=3.3 mM for 1,2-dioleoyl-3-(pyren-1-yl)-decanoyl-rac-glycerol (DPG) ; Vmax=0.041 umol/min/mg enzyme for esterase activity using PNB as a substrate ; Vmax=1 umol/min/mg enzyme for triacylglycerol lipase activity using DPG as a substrate ;
Catalytic ActivityTriacylglycerol + H(2)O = diacylglycerol + a carboxylate.
Disruption PhenotypeLeads to the appearance of giant lipid droplets and an excessive accumulation of non-polar lipids and phospholipids upon growth on medium containing oleic acid as a sole carbon source
Disruption PhenotypeLeads to the appearance of giant lipid droplets and an excessive accumulation of non-polar lipids and phospholipids upon growth on medium containing oleic acid as a sole carbon source. {ECO:0000269|PubMed:21478434}.
FunctionSerine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis. Exhibits both esterase and triacylglycerol lipase activity
FunctionSerine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis. Exhibits both esterase and triacylglycerol lipase activity. {ECO:0000269|PubMed:21478434}.
SimilarityBelongs to the AB hydrolase superfamily. Lipase family
SimilarityBelongs to the AB hydrolase superfamily. Lipase family. {ECO:0000305}.
Subcellular LocationLipid droplet .
Subcellular LocationLipid droplet {ECO:0000269|PubMed:21478430}.