LMP007249 UniProt Annotations
Gene Name phosphoprotein phosphatase CDC14 Protein Entry CDC14_YEAST UniProt ID Q00684 Species Yeast (S288c)
Comment type Description Biophysicochemical Properties Kinetic parameters: KM=4 mM for p-nitrophenyl phosphate {ECO:0000269|PubMed:9295359}; pH dependence: Optimum pH is 6.9. {ECO:0000269|PubMed:9295359}; Biophysicochemical Properties Kinetic parameters: KM=4 mM for p-nitrophenyl phosphate ; pH dependence: Optimum pH is 6.9. ; Catalytic Activity Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. {ECO:0000255|PROSITE-ProRule:PRU10044, ECO:0000269|PubMed:15128740, ECO:0000269|PubMed:9295359}. Function Protein phosphatase which antagonizes mitotic cyclin- dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Inhibits transcription of ribosomal genes (rDNA) during anaphase and controls segregation of nucleolus by facilitating condensin targeting to rDNA chromatin in anaphase. Dephosphorylates SIC1, a CDC28 inhibitor, and SWI5, a transcription factor for SIC1, and induces degradation of mitotic cyclins, likely by dephosphorylating the activator of mitotic cyclin degradation, CDH1. Dephosphorylates the microtubule bundling factor ASE1 which is required to define a centered and focused mitotic spindle midzone that can drive continuous spindle elongation. Dephosphorylates the anaphase-promoting complex inhibitor ACM1, leading to its degradation. Facilitates INN1-CYK3 complex formation which promotes cytokinesis through the dephosphosprylation of CDC28-phosphosphorylated INN1. Reverts also the inhibitory CDC28 phosphorylation of CHS2 for endoplasmic reticulum export, ensuring that septum formation is contingent upon chromosome separation and exit from mitosis. Additional substrates for CDC14 are the formins BNI1 and BNR1, as well as CDC6, DBP2, DSN1, INCENP, KAR9, MCM3, ORC2, ORC6, SLD2, and SWI6. Activity is inhibited by interaction with NET1 which sequesters it to the nucleolus. {ECO:0000269|PubMed:11511359, ECO:0000269|PubMed:12737806, ECO:0000269|PubMed:14993267, ECO:0000269|PubMed:15004526, ECO:0000269|PubMed:15137939, ECO:0000269|PubMed:15190202, ECO:0000269|PubMed:15917648, ECO:0000269|PubMed:17116692, ECO:0000269|PubMed:18235228, ECO:0000269|PubMed:18287090, ECO:0000269|PubMed:18595708, ECO:0000269|PubMed:19158678, ECO:0000269|PubMed:19339280, ECO:0000269|PubMed:20619650, ECO:0000269|PubMed:20660629, ECO:0000269|PubMed:20923974, ECO:0000269|PubMed:20980394, ECO:0000269|PubMed:21127052, ECO:0000269|PubMed:21690308, ECO:0000269|PubMed:21784165, ECO:0000269|PubMed:22072794, ECO:0000269|PubMed:22078879, ECO:0000269|PubMed:22117071, ECO:0000269|PubMed:22363215, ECO:0000269|PubMed:22454527, ECO:0000269|PubMed:22872148, ECO:0000269|PubMed:7021319, ECO:0000269|PubMed:8668128, ECO:0000269|PubMed:9295359, ECO:0000269|PubMed:9885559}. Interaction P35734:ASK1; NbExp=2; IntAct=EBI-4192, EBI-26682; Q01389:BCK1; NbExp=4; IntAct=EBI-4192, EBI-3470; P38041:BOI1; NbExp=2; IntAct=EBI-4192, EBI-3719; P53894:CBK1; NbExp=2; IntAct=EBI-4192, EBI-4110; P32562:CDC5; NbExp=2; IntAct=EBI-4192, EBI-4440; P38147:CHK1; NbExp=2; IntAct=EBI-4192, EBI-4593; P15790:CKA1; NbExp=3; IntAct=EBI-4192, EBI-9533; P19454:CKA2; NbExp=3; IntAct=EBI-4192, EBI-9548; P24870:CLB3; NbExp=2; IntAct=EBI-4192, EBI-4522; Q03898:FIN1; NbExp=2; IntAct=EBI-4192, EBI-32941; P53233:FMP48; NbExp=2; IntAct=EBI-4192, EBI-9664; P38785:GIC1; NbExp=2; IntAct=EBI-4192, EBI-7575; P21965:MCK1; NbExp=2; IntAct=EBI-4192, EBI-10517; Q02196:MET14; NbExp=2; IntAct=EBI-4192, EBI-9485; P47035:NET1; NbExp=13; IntAct=EBI-4192, EBI-25953; P38826:ORC6; NbExp=2; IntAct=EBI-4192, EBI-12588; P39940:RSP5; NbExp=2; IntAct=EBI-4192, EBI-16219; P38990:SAK1; NbExp=2; IntAct=EBI-4192, EBI-12863; P38634:SIC1; NbExp=2; IntAct=EBI-4192, EBI-17127; P06700:SIR2; NbExp=5; IntAct=EBI-4192, EBI-17219; P38283:SLI15; NbExp=2; IntAct=EBI-4192, EBI-20842; P06782:SNF1; NbExp=2; IntAct=EBI-4192, EBI-17516; P06784:STE7; NbExp=2; IntAct=EBI-4192, EBI-18389; P32944:SWE1; NbExp=3; IntAct=EBI-4192, EBI-18607; Q02208:TOF2; NbExp=8; IntAct=EBI-4192, EBI-27048; Q03785:VHS1; NbExp=2; IntAct=EBI-4192, EBI-35568; Miscellaneous Present with 8550 molecules/cell in log phase SD medium Miscellaneous Present with 8550 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. Sequence Caution Sequence=AAA34477.1; Type=Frameshift; Positions=116, 190; Evidence={ECO:0000305}; Sequence=CAA52971.1; Type=Frameshift; Positions=117; Evidence={ECO:0000305}; Sequence Caution Sequence=AAA34477.1; Type=Frameshift; Positions=116, 190; Evidence= ; Sequence=CAA52971.1; Type=Frameshift; Positions=117; Evidence= ; Similarity Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily Similarity Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. {ECO:0000305}. Subcellular Location Nucleus, nucleolus. Cytoplasm. Bud neck. Note=Sequestered in the nucleolus for most of the cell cycle by the nucleolar proteins NET1 and TOF2, and is released into the nucleus and cytoplasm during anaphase. CDC55 maintains CDC14 sequestration in the nucleolus during early meiosis, which is essential for the assembly of the meiosis I spindle. In anaphase, the CDC14 early anaphase release (FEAR) network (including CDC5, ESP1, and SLK19), and the mitotic exit network (including the DBF2-MOB1 complex) coordinately trigger the release of CDC14 from the nucleolus. Subunit Component of the RENT (regulator of nucleolar silencing and telophase) complex which is composed of at least NET1, CDC14 and SIR2. Interacts with CDC5, CRM1, SIC1, TOF2 and UTP7. {ECO:0000269|PubMed:11511359, ECO:0000269|PubMed:15917648, ECO:0000269|PubMed:18595708, ECO:0000269|PubMed:18794331, ECO:0000269|PubMed:18927509, ECO:0000269|PubMed:21127052, ECO:0000269|PubMed:9885559}.
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