LIPID MAPS® Gene/Proteome Database (LMPD)

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LMPD Record

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LMP006991

UniProt Annotations

Entry Information
Gene NameOst2p
Protein EntryOST2_YEAST
UniProt IDP46964
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityDolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
FunctionEssential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
InteractionP52870:SBH1; NbExp=2; IntAct=EBI-12673, EBI-16410; P32915:SEC61; NbExp=2; IntAct=EBI-12673, EBI-16400;
PathwayProtein modification; protein glycosylation.
Sequence CautionSequence=CAA64024.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAA99300.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Sequence CautionSequence=CAA64024.1; Type=Erroneous initiation; Evidence= ; Sequence=CAA99300.1; Type=Erroneous initiation; Evidence= ;
SimilarityBelongs to the DAD/OST2 family
SimilarityBelongs to the DAD/OST2 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein.
SubunitComponent of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST3, OST4, OST5, OST6, WBP1 and SWP1. Interacts with SEC61 and SSS1. {ECO:0000269|PubMed:15831493, ECO:0000269|PubMed:15886282, ECO:0000269|PubMed:8175708, ECO:0000269|PubMed:9405463}.