LIPID MAPS

4beta-hydroxy-cholesterol

LMST01010014

SciEx

[M+NH4]+

4beta-hydroxy-cholesterol

LMST01010014

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

4beta-hydroxy-cholesterol

LMID

LMST01010014

Systematic name

cholest-5-en-3beta,4beta-diol

m/z

385.3465

Adduct / Ion

[M+H-H2O]+

Charge

1

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

10,20,30

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

8.82

DOI for method

Date acquired

December 2025

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Cayman Chemical

4beta-hydroxy-cholesterol

LMST01010014

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

4beta-hydroxy-cholesterol

LMID

LMST01010014

Systematic name

cholest-5-en-3beta,4beta-diol

m/z

385.3465

Adduct / Ion

[M+H-H2O]+

Charge

1

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

20,30,40

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

8.82

DOI for method

Date acquired

December 2025

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Cayman Chemical

4beta-hydroxy-cholesterol

LMST01010014

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

4beta-hydroxy-cholesterol

LMID

LMST01010014

Systematic name

cholest-5-en-3beta,4beta-diol

m/z

385.3465

Adduct / Ion

[M+H-H2O]+

Charge

1

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

30,40,50

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

8.82

DOI for method

Date acquired

December 2025

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Cayman Chemical

4beta-hydroxy-cholesterol

LMST01010014

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

4beta-hydroxy-cholesterol

LMID

LMST01010014

Systematic name

cholest-5-en-3beta,4beta-diol

m/z

385.3465

Adduct / Ion

[M+H-H2O]+

Charge

1

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

75

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

8.82

DOI for method

Date acquired

February 2026

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Cayman Chemical

4beta-hydroxy-cholesterol

LMST01010014

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

4beta-hydroxy-cholesterol

LMID

LMST01010014

Systematic name

cholest-5-en-3beta,4beta-diol

m/z

385.3465

Adduct / Ion

[M+H-H2O]+

Charge

1

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

80

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

8.82

DOI for method

Date acquired

February 2026

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Cayman Chemical

4beta-hydroxy-cholesterol

LMST01010014

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

4beta-hydroxy-cholesterol

LMID

LMST01010014

Systematic name

cholest-5-en-3beta,4beta-diol

m/z

385.3465

Adduct / Ion

[M+H-H2O]+

Charge

1

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

90

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

8.82

DOI for method

Date acquired

February 2026

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Cayman Chemical