Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets
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Legend: | : View data for technical replicates : Graph of technical replicates(within an expt.) : Graph of biological replicates(across expts.) |
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Details/Graph technical replicates | LM_ID | Name | Mass (neutral) |
WT-Fat WT-Chow KO-Fat KO-Chow |
Units . . . . | |
---|---|---|---|---|---|---|
(MSJ070927) | LMST01010086 | 22R-hydroxy-cholesterol | 402.3 (ions) | 6.8 - 15 6.7 |
pmol/1E6 cells | |
(MSJ070927) | LMST01010018 | 25-hydroxy-cholesterol | 402.3 (ions) | 1.8 - 4.3 1.7 |
pmol/1E6 cells | |
(MSJ070927) | LMST01010057 | 27-hydroxy-cholesterol | 402.3 (ions) | 1 - 2 3.3 |
pmol/1E6 cells | |
(MSJ070927) | LMST01010014 | 4beta-hydroxy-cholesterol | 402.3 (ions) | 3.5 4 4.2 5.2 |
pmol/1E6 cells | |
(MSJ070927) | LMST01010013 | 7alpha-hydroxy-cholesterol | 402.3 (ions) | 6.9 3.3 11 5.3 |
pmol/1E6 cells | |
(MSJ070927) | LMST01010069 | 7-Dehydrocholesterol | 384.3 (ions) | - - - - |
pmol/1E6 cells | |
(MSJ070927) | LMST01010049 | 7-oxo-cholesterol | 400.3 (ions) | - - - - |
pmol/1E6 cells | |
(MSJ070927) | LMST01010015 | Cholestenone | 384.3 (ions) | 0.7 0.53 - - |
pmol/1E6 cells | |
(MSJ070927) | LMST01010001 | Cholesterol | 386.4 (ions) | 9890 8860 32720 13780 |
pmol/1E6 cells | |
(MSJ070927) | LMST01010017 | Lanosterol | 426.4 (ions) | 3.3 2.5 5 2.5 |
pmol/1E6 cells |
*:
The units in the table and graphs correspond to the pmol of each species which was determined by dividing
the areas under the LC MS/MS elution curve for each species by that for an appropriate deuterated surrogate
standard then multiplied by the amount of the surrogate standard added (~10 pmol to 2 nmol depending on the
compound of interest). For most of the analytes, the intensities were similiar for equivalent amounts of the
analyte as for the internal standard when the LC MS/MS parameters were optimized and quantitative analysis
was conducted by Multiple Reaction Monitoring (MRM). If necessary, small corrections were made using
empirically determined relative response factors (RRF). The pmoles were then normalized to the measured
quantity of DNA present in each sample (i.e. dish of RAW cells at time zero or at varying times
following treatment with and without Kdo2 lipid A).