LMPD Database

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LMP013012

UniProt Annotations

Entry Information
Gene Namearachidonate 12-lipoxygenase
Protein Entry
UniProt ID
SpeciesRat
Comments
Comment typeDescription
Catalytic Activity(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa- 7,9,11,14-tetraenoate + H(2)O = (5S,14R,15S)-trihydroxy- (6E,8Z,10E,12E)-eicosatetraenoate. {ECO:0000269|PubMed:23382512}.
Catalytic Activity(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa- 7,9,11,14-tetraenoate + H(2)O = (5S,6R,15S)-trihydroxy- (7E,9E,11Z,13E)-eicosatetraenoate. {ECO:0000269|PubMed:23382512}.
Catalytic ActivityArachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate. {ECO:0000269|PubMed:23382512}.
CofactorName=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726}; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Enzyme RegulationActivated by EGF. {ECO:0000250}.
FunctionNon-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269|PubMed:15105833, ECO:0000269|PubMed:23382512}.
PathwayLipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. {ECO:0000269|PubMed:23382512}.
SimilarityBelongs to the lipoxygenase family. {ECO:0000305}.
SimilarityContains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
SimilarityContains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Subcellular LocationCytoplasm, cytosol. Membrane {ECO:0000250}. Note=Membrane association is stimulated by EGF. {ECO:0000250}.