LMPD Database

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LMP012858

UniProt Annotations

Entry Information
Gene NameN-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Protein EntryASAH2_RAT
UniProt IDQ91XT9
SpeciesRat
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=71.4 uM for octanoyl-sphingosine {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=66 uM for palmitoyl-sphingosine {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=1.29 M for C16-ceramide {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=3.84 M for dihydroceramide {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=160 umol/min/mg enzyme with octanoyl-sphingosine as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=16 umol/min/mg enzyme with palmitoyl-sphingosine as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=4.4 umol/min/mg enzyme with C16-ceramide as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=1.2 umol/min/mg enzyme with dihydroceramide as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; pH dependence: Optimum pH is 7-10. {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782};
Catalytic ActivityN-acylsphingosine + H(2)O = a carboxylate + sphingosine. {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816}.
Enzyme RegulationInhibited by dithiothreitol (DTT), 2- mercaptoethanol, Zn(2+) and Cu(2+).
FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract
InductionBy interleukin-1-beta in renal mesengial cells
PtmN-glycosylated. Required for enzyme activity.
PtmO-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
PtmPhosphorylated. May prevent ubiquitination and subsequent degradation. {ECO:0000269|PubMed:12359735, ECO:0000269|PubMed:12482609}.
PtmUbiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid
SimilarityBelongs to the neutral ceramidase family
Subcellular LocationCell membrane {ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:12499379}; Single-pass type II membrane protein . Note=The neutral ceramidase soluble form is a secreted protein.
Tissue SpecificityWidelky expressed. Highly expressed in brain, kidney and heart. Expressed at lower level in other tissues such as liver. Localizes in the epithelia of the jejunum and ileum