LMPD Database

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LMP012728

UniProt Annotations

Entry Information
Gene Nameradical S-adenosyl methionine domain containing 2
Protein EntryRSAD2_RAT
UniProt IDO70600
SpeciesRat
Comments
Comment typeDescription
CofactorName=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence= ; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. ;
Developmental StageNot detected in undifferentiated primary osteoblasts. Expression increases during differentiation and declines to much reduced levels in mature osteoblasts
DomainThe N-terminal region (1-41) is necessary for its localization to the endoplasmic reticulum membrane and lipid droplet
FunctionInterferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of viruses, including west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus and vesicular stomatitis virus (VSV). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins (By similarity)
InductionBy interferon type I, type II and LPS. Interferon alpha induction is rapid and transient, peaks 4-6 hours after stimulation and returns to basal levels 24 hours after stimulation. Interferon gamma elicits a more prolonged response where expression remains elevated 48 hours after stimulation. Induced by infection with Vesicular stomatitis virus and pseudorabies virus, presumably through type I interferon pathway
SimilarityBelongs to the radical SAM superfamily. RSAD2 family
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane protein ; Cytoplasmic side {ECO:0000250}. Lipid droplet .
SubunitHomodimer. Interacts (via C-terminus) with VAPA/VAP33 (via C-terminus) and inhibits its interaction with hepatitis virus C (HCV) protein NS5A. Interacts with HADHB, IRAK1 and TRAF6 (By similarity)
Tissue SpecificityIn neonatal rat tibia, specifically localized in cells of the periosteum, in osteoblasts lining endosteal and peristeal bone surfaces, to articular surfaces of cartilage and in perichondral cells but not in chondrocytes (at protein level). Expressed predominantly in bone marrow and spleen