LMPD Database

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LMP011316

UniProt Annotations

Entry Information
Gene Namesterol 14-demethylase
Protein EntryCP511_ARATH
UniProt IDQ9SAA9
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityA 14-alpha-methylsteroid + 3 O(2) + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP(+) + 4 H(2)O. {ECO:0000269|PubMed:11437378, ECO:0000269|PubMed:16040657}.
CofactorName=heme; Xref=ChEBI:CHEBI:30413;
Disruption PhenotypeLack of membrane integrity. Seedling lethality. {ECO:0000269|PubMed:15266054, ECO:0000269|PubMed:16040657, ECO:0000269|PubMed:16169959}.
FunctionInvolved in sterol biosynthesis. Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta- 8,14,24(28)-trien-3 beta-ol. {ECO:0000269|PubMed:11437378, ECO:0000269|PubMed:16040657, ECO:0000269|PubMed:16169959}.
MiscellaneousDecreased expression of CYP51G1 by antisense leads to a semidwarf phenotype in the early growth stage and a longer life span. Disruption mutants accumulate obtusifoliol and 14- alpha-methyl-sterols and cannot be rescued by exogenous application of brassinosteroids.
SimilarityBelongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular LocationMembrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Tissue SpecificityExpressed in leaves, roots, stems, siliques, flowers, flower buds and seedlings. {ECO:0000269|PubMed:16040657}.