LMPD Database

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LMP011290

UniProt Annotations

Entry Information
Gene Namesolanesyl diphosphate synthase 2
Protein EntrySPS2_ARATH
UniProt IDQ76FS5
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=6.89 uM for farnesyl diphosphate (in the presence of 500 uM of isopentenyl diphosphate) {ECO:0000269|PubMed:15784989}; KM=0.843 uM for geranylgeranyl diphosphate (in the presence of 100 uM of isopentenyl diphosphate) {ECO:0000269|PubMed:15784989}; KM=182 uM for isopentenyl diphosphate (in the presence of 20 uM of farnesyl diphosphate) {ECO:0000269|PubMed:15784989}; KM=28.9 uM for isopentenyl diphosphate (in the presence of 4 uM of geranylgeranyl diphosphate) {ECO:0000269|PubMed:15784989}; Note=kcat is 3.77 sec(-1) with farnesyl diphosphate as substrate (in the presence of 500 uM of isopentenyl diphosphate). kcat is 2.33 sec(-1) with geranylgeranyl diphosphate as substrate (in the presence of 100 uM of isopentenyl diphosphate). kcat is 2.83 sec(-1) with isopentenyl diphosphate as substrate (in the presence of 20 uM of farnesyl diphosphate). kcat is 1.72 sec(-1) with isopentenyl diphosphate as substrate (in the presence of 4 uM of geranylgeranyl diphosphate).; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:15784989};
Catalytic ActivityGeranylgeranyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-nonaprenyl diphosphate. {ECO:0000269|PubMed:15784989}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
FunctionInvolved in providing solanesyl diphosphate for plastoquinone-9 formation. Prefers geranylgeranyl diphosphate to farnesyl diphosphate as substrate. No activity with geranyl diphosphate or dimethylallyl diphosphate as substrate. {ECO:0000269|PubMed:15653808, ECO:0000269|PubMed:15784989}.
Sequence CautionSequence=AAD50025.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the FPP/GGPP synthase family. {ECO:0000305}.
Subcellular LocationPlastid, chloroplast {ECO:0000269|PubMed:15653808, ECO:0000269|PubMed:15784989}.
SubunitHomodimer. {ECO:0000250}.
Tissue SpecificityHigher expression in leaves than in roots. {ECO:0000269|PubMed:15784989}.