LMPD Database

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LMP010825

UniProt Annotations

Entry Information
Gene NameMyotubularin 2
Protein EntryMYTM2_ARATH
UniProt IDF4JWB3
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=216.5 uM for PtdIns3P {ECO:0000269|PubMed:22324391}; KM=158.2 uM for PtdIns3,5P(2) {ECO:0000269|PubMed:22324391}; Vmax=15.4 pmol/min/mg enzyme with PtdIns3P as substrate {ECO:0000269|PubMed:22324391}; Vmax=28.4 pmol/min/mg enzyme with PtdIns3,5P(2) as substrate {ECO:0000269|PubMed:22324391};
Catalytic Activity1-phosphatidyl-1D-myo-inositol 3,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate. {ECO:0000269|PubMed:22324391}.
Catalytic Activity1-phosphatidyl-1D-myo-inositol 3-phosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol + phosphate. {ECO:0000269|PubMed:22324391}.
Developmental StageExpressed in young seedlings, especially at the tip of the growing shoot meristems. Later observed in roots and in aerial parts. Weakly expressed in leaves with local higher levels in the trichomes and in cotyledon veins. Present at low levels in flowers with higher accumulation in cells at organ-stem junctions. Restricted to the developing peduncle. {ECO:0000269|PubMed:22324391}.
Disruption PhenotypeNo visible phenotype in both normal and dehydration conditions. {ECO:0000269|PubMed:22324391}.
FunctionPhosphatase with phosphoinositide 3'-phosphatase activity that can use phosphatidylinositol-3-phosphate (PtdIns3P) and phosphatidylinositol-3,5-diphosphate (PtdIns3,5P(2)) as substrates and produces phosphatidylinositol-5-phosphate (PtdIns5P); participates in pathway(s) that transfer gene regulatory signals to the nucleus. {ECO:0000269|PubMed:22324391}.
Sequence CautionSequence=CAB85561.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. {ECO:0000305}.
SimilarityContains 1 GRAM domain. {ECO:0000305}.
SimilarityContains 1 myotubularin phosphatase domain. {ECO:0000255|PROSITE-ProRule:PRU00669}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:22324391}. Note=Highly concentrated at the peripheral lobes of the epidermal cells.
Tissue SpecificityMostly expressed in flowers and roots, and, to a lower extent, in siliques and leaves. {ECO:0000269|PubMed:22324391}.