LMPD Database

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LMP009636

UniProt Annotations

Entry Information
Gene Name1,8-cineole synthase
Protein EntryCIN1_ARATH
UniProt IDP0DI77
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P0DI76-1; Sequence=Displayed; Name=2; IsoId=P0DI76-2; Sequence=VSP_035149, VSP_035150; Note=May be due to intron retention. No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=0.2 uM for geranyl diphosphate {ECO:0000269|PubMed:15299125}; Vmax=10.9 pmol/sec/mg enzyme toward geranyl diphosphate {ECO:0000269|PubMed:15299125}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:15299125};
Catalytic ActivityGeranyl diphosphate + H(2)O = 1,8-cineole + diphosphate. {ECO:0000269|PubMed:15299125}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
DomainThe Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+).
FunctionInvolved in monoterpene (C10) biosynthesis. The major product is 1,8-cineole (52%) followed by minor amounts of sabinene (14.5%), myrcene (13.3%), (-)-(1S)-beta-pinene (7.8%), (-)-(4S)- limonene (4.0%), (E)-beta-ocimene (2.7%), alpha-terpineol (2.4%), (-)-(1S)-alpha-pinene (1.9%), terpinolene (0.8%), and (+)-alpha- thujene (0.6%). {ECO:0000269|PubMed:15299125}.
InductionNot induces in aerial parts by treatments with jasmonic acid, 6-ethyl indanoyl-L-Ile, fungal peptaibol elicitor alamethicin or herbivory. {ECO:0000269|PubMed:15299125}.
PathwaySecondary metabolite biosynthesis; terpenoid biosynthesis.
SimilarityBelongs to the terpene synthase family. Tpsb subfamily. {ECO:0000305}.
Subcellular LocationPlastid, chloroplast {ECO:0000305}.
Tissue SpecificityPredominantly expressed in roots and at much lower levels in siliques. Not found in leaves, flowers or stems. Also detected in flowers in cv. Landsberg erecta. Not expressed in root apical meristem and elongation zone. Found in the vascular system of young roots and additionally in the cortex and epidermal cell layer of older roots. {ECO:0000269|PubMed:12566586, ECO:0000269|PubMed:15299125}.