LMPD Database

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LMP007674

UniProt Annotations

Entry Information
Gene NameGDP-mannose mannosyl hydrolase
Protein EntryGMM_ECOLI
UniProt IDP32056
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.3 mM for GDP-mannose {ECO:0000269|PubMed:7592609}; KM=1.9 mM for GDP-glucose {ECO:0000269|PubMed:7592609}; Vmax=1.6 umol/min/mg enzyme with GDP-mannose as substrate {ECO:0000269|PubMed:7592609}; Vmax=7.5 umol/min/mg enzyme with GDP-glucose as substrate {ECO:0000269|PubMed:7592609}; Note=GDP-alpha-D-mannose is likely to be the biological substrate, but the Kcat/KM obtained with GDP-alpha-D-glucose is very similar to that with GDP-alpha-D-mannose.; pH dependence: Optimum pH is 9.3. {ECO:0000269|PubMed:7592609};
Catalytic ActivityGDP-D-mannose + H(2)O = GDP + D-mannose. {ECO:0000255|HAMAP-Rule:MF_00941, ECO:0000269|PubMed:10913267, ECO:0000269|PubMed:7592609}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00941, ECO:0000269|PubMed:10913267, ECO:0000269|PubMed:12196023, ECO:0000269|PubMed:15274914}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP- Rule:MF_00941, ECO:0000269|PubMed:10913267, ECO:0000269|PubMed:12196023, ECO:0000269|PubMed:15274914};
FunctionHydrolyzes both GDP-mannose and GDP-glucose. Could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. Might also be involved in the biosynthesis of the slime polysaccharide colanic acid. {ECO:0000269|PubMed:10913267, ECO:0000269|PubMed:7592609}.
Mass SpectrometryMass=18472; Mass_error=67; Method=MALDI; Range=1-159; Evidence={ECO:0000269|PubMed:10913267};
Sequence CautionSequence=AAC77844.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
SimilarityBelongs to the Nudix hydrolase family. {ECO:0000255|HAMAP-Rule:MF_00941}.
SimilarityContains 1 nudix hydrolase domain. {ECO:0000255|HAMAP- Rule:MF_00941}.
SubunitHomodimer. {ECO:0000255|HAMAP-Rule:MF_00941, ECO:0000269|PubMed:10913267, ECO:0000269|PubMed:15274914, ECO:0000269|PubMed:16981689}.