LMPD Database

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LMP007510

UniProt Annotations

Entry Information
Gene NameUbx2p
Protein EntryUBX2_YEAST
UniProt IDQ04228
SpeciesYeast (S288c)
Comments
Comment typeDescription
FunctionIntegral endoplasmic reticulum membrane protein that coordinates the assembly of the ER-associated protein degradation (ERAD) machinery at the ER membrane. Mediates binding of CDC48 to the E3 ubiquitin ligases SSM4/DOA10 and HRD1, and to ERAD substrates. Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Also component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome. {ECO:0000269|PubMed:15258615, ECO:0000269|PubMed:16179952, ECO:0000269|PubMed:16179953, ECO:0000269|PubMed:16873066}.
InteractionP25694:CDC48; NbExp=11; IntAct=EBI-27730, EBI-4308; P40318:SSM4; NbExp=5; IntAct=EBI-27730, EBI-18208; P53044:UFD1; NbExp=5; IntAct=EBI-27730, EBI-19997; Q99220:YOS9; NbExp=3; IntAct=EBI-27730, EBI-34938;
MiscellaneousPresent with 12600 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
SimilarityContains 1 UBX domain. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
SubunitComponent of the DOA10 complex which contains SSM4/DOA10, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the membrane integral E3 ligase SSM4/DOA10 and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recuited by UBX2/SEL1. Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, KAR2, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factors KAR2 and YOS9, and the heterotrimeric UFD1- NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with CDC48. {ECO:0000269|PubMed:15258615, ECO:0000269|PubMed:16179952, ECO:0000269|PubMed:16179953, ECO:0000269|PubMed:16873066}.