LMPD Database

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LMP007504

UniProt Annotations

Entry Information
Gene Nameacetolactate synthase catalytic subunit
Protein EntryILVB_YEAST
UniProt IDP07342
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic Activity2 pyruvate = 2-acetolactate + CO(2).
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) ion per subunit. ;
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000250};
CofactorName=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
Enzyme RegulationThe regulatory subunit ILV6 stimulates enzymatic activity seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP
Enzyme RegulationThe regulatory subunit ILV6 stimulates enzymatic activity seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP. {ECO:0000269|PubMed:10213630}.
MiscellaneousContains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry.
MiscellaneousPresent with 31900 molecules/cell in log phase SD medium
MiscellaneousPresent with 31900 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayAmino-acid biosynthesis; L-isoleucine biosynthesis; L- isoleucine from 2-oxobutanoate: step 1/4.
PathwayAmino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
SimilarityBelongs to the TPP enzyme family
SimilarityBelongs to the TPP enzyme family. {ECO:0000305}.
Subcellular LocationMitochondrion.
SubunitHomodimer. The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6
SubunitHomodimer. The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6. {ECO:0000269|PubMed:12496246}.