LMPD Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
LMP007433

UniProt Annotations

Entry Information
Gene Namephosphatidylethanolamine N-methyltransferase
Protein EntryCHO2_YEAST
UniProt IDP05374
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=60 uM for S-adenosyl-L-methionine {ECO:0000269|PubMed:2684666}; pH dependence: Optimum pH is 9.9. {ECO:0000269|PubMed:2684666};
Biophysicochemical PropertiesKinetic parameters: KM=60 uM for S-adenosyl-L-methionine ; pH dependence: Optimum pH is 9.9. ;
Catalytic ActivityS-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.
FunctionCatalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis. Preferentially converts di-C16:1 substrates. {ECO:0000269|PubMed:15258140, ECO:0000269|PubMed:2684666, ECO:0000269|PubMed:2850468, ECO:0000269|PubMed:3025587, ECO:0000269|PubMed:3066687, ECO:0000269|PubMed:6759124, ECO:0000269|PubMed:6988218}.
InductionRepressed by myo-inositol and choline
InductionRepressed by myo-inositol and choline. {ECO:0000269|PubMed:1650774, ECO:0000269|PubMed:1954254}.
InteractionP25376:AGP1; NbExp=1; IntAct=EBI-2049142, EBI-2357; P08456:CHO1; NbExp=1; IntAct=EBI-2049142, EBI-14055; P39540:ELO1; NbExp=1; IntAct=EBI-2049142, EBI-2049096; P10614:ERG11; NbExp=1; IntAct=EBI-2049142, EBI-5127; P41543:OST1; NbExp=1; IntAct=EBI-2049142, EBI-12651; P40975:PMP2; NbExp=1; IntAct=EBI-2049142, EBI-2043041; Q04947:RTN1; NbExp=1; IntAct=EBI-2049142, EBI-38020; P32368:SAC1; NbExp=1; IntAct=EBI-2049142, EBI-16210; P39986:SPF1; NbExp=1; IntAct=EBI-2049142, EBI-3128; P38800:YHR080C; NbExp=1; IntAct=EBI-2049142, EBI-24597;
MiscellaneousPresent with 1810 molecules/cell in log phase SD medium
MiscellaneousPresent with 1810 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis.
SimilarityBelongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family
SimilarityBelongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family. {ECO:0000305}.
Subcellular LocationMembrane; Multi-pass membrane protein.