LMPD Database

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LMP007415

UniProt Annotations

Entry Information
Gene Namesterol 14-demethylase
Protein EntryCP51_YEAST
UniProt IDP10614
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityA 14-alpha-methylsteroid + 3 O(2) + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP(+) + 4 H(2)O.
CofactorName=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
FunctionCatalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
InteractionP53045:ERG25; NbExp=3; IntAct=EBI-5127, EBI-6506;
MiscellaneousIt is the main target for antifungal compounds of the triazole family like ketoconazole which inhibits by coordinating the iron atom at the sixth ligand position.
MiscellaneousPresent with 73200 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwaySteroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
SimilarityBelongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular LocationMembrane; Single-pass membrane protein.
SubunitInteracts with ERG28. {ECO:0000269|PubMed:15995173}.