LMPD Database

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LMP007406

UniProt Annotations

Entry Information
Gene NameOst3p
Protein EntryOST3_YEAST
UniProt IDP48439
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityDolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
FunctionEssential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
InteractionQ99380:OST4; NbExp=2; IntAct=EBI-12680, EBI-12689; P39007:STT3; NbExp=2; IntAct=EBI-12680, EBI-18447;
MiscellaneousPresent with 4030 molecules/cell in log phase SD medium
MiscellaneousPresent with 4030 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the OST3/OST6 family
SimilarityBelongs to the OST3/OST6 family. {ECO:0000305}.
SimilarityContains 1 thioredoxin domain
SimilarityContains 1 thioredoxin domain. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein .
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
SubunitComponent of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST3, OST4 and STT3 probably form a subcomplex. May interact directly with OST1, OST2, OST4 and STT3. {ECO:0000269|PubMed:8175708, ECO:0000269|PubMed:9405463}.