LMPD Database

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LMP007404

UniProt Annotations

Entry Information
Gene Nameacetate--CoA ligase 1
Protein EntryACS1_YEAST
UniProt IDQ01574
SpeciesYeast (S288c)
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.32 mM for acetate {ECO:0000269|PubMed:8910545}; KM=1.4 mM for ATP {ECO:0000269|PubMed:8910545}; Vmax=1.1 umol/min/mg enzyme {ECO:0000269|PubMed:8910545};
Catalytic ActivityATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.
CautionIt is uncertain whether Met-1 or Met-25 is the initiator. {ECO:0000305}.
FunctionCatalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. "Aerobic" isozyme of acetyl-coenzyme A synthetase, which supports growth on nonfermentable carbon sources such as glycerol and ethanol. May be required for assimilation of ethanol and acetate. {ECO:0000269|PubMed:16857587, ECO:0000269|PubMed:8910545}.
InductionBy acetate, acetaldehyde and ethanol. Subject to glucose catabolite repression. Inactivated and degraded after addition of glucose (at protein level). {ECO:0000269|PubMed:8910545, ECO:0000269|PubMed:9252575}.
InteractionQ02821:SRP1; NbExp=1; IntAct=EBI-2128, EBI-1797; P80235:YAT1; NbExp=1; IntAct=EBI-2128, EBI-3928; Q12110:YLR049C; NbExp=1; IntAct=EBI-2128, EBI-33635;
MiscellaneousPresent with 2890 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
SimilarityBelongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular LocationMicrosome {ECO:0000305}. Cytoplasm. Mitochondrion. Nucleus.