LMPD Database

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LMP007387

UniProt Annotations

Entry Information
Gene Namephosphoprotein phosphatase PP4 catalytic subunit PPH3
Protein EntryPP4C_YEAST
UniProt IDP32345
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic Activity[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate.
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ;
FunctionForms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. PPH3 is directly involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability
FunctionForms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. PPH3 is directly involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability. {ECO:0000269|PubMed:10940301, ECO:0000269|PubMed:16299494}.
InteractionP38193:PSY4; NbExp=8; IntAct=EBI-12759, EBI-21239;
MiscellaneousPresent with 2840 molecules/cell in log phase SD medium
MiscellaneousPresent with 2840 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PtmReversibly methyl esterified on Leu-308 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity)
PtmReversibly methyl esterified on Leu-308 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity). {ECO:0000250}.
SimilarityBelongs to the PPP phosphatase family. PP-4 (PP-X) subfamily
SimilarityBelongs to the PPP phosphatase family. PP-4 (PP-X) subfamily. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}.
Subcellular LocationCytoplasm . Nucleus .
SubunitCatalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4. Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with SPT5 and TAP42. {ECO:0000269|PubMed:14551259, ECO:0000269|PubMed:15447631, ECO:0000269|PubMed:16085932, ECO:0000269|PubMed:16299494}.