LMPD Database

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LMP007371

UniProt Annotations

Entry Information
Gene NameVps15p
Protein EntryVPS15_YEAST
UniProt IDP22219
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityATP + a protein = ADP + a phosphoprotein.
DomainTruncation of 30 residues from the C-terminus results in a temperature-conditional defect in protein sorting.
FunctionSerine/threonine-protein kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Is also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). By regulating VPS34 kinase activity, VPS15 appears to be essential for the efficient delivery of soluble hydrolases to the yeast vacuole. {ECO:0000269|PubMed:11157979, ECO:0000269|PubMed:12244127, ECO:0000269|PubMed:1756716, ECO:0000269|PubMed:1988155, ECO:0000269|PubMed:3062374, ECO:0000269|PubMed:7989323, ECO:0000269|PubMed:8387919, ECO:0000269|PubMed:8509446, ECO:0000269|PubMed:8649377}.
InteractionP08539:GPA1; NbExp=2; IntAct=EBI-20347, EBI-7376;
MiscellaneousPresent with 279 molecules/cell in log phase SD medium
MiscellaneousPresent with 279 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PtmAutophosphorylated.
SimilarityBelongs to the protein kinase superfamily. Ser/Thr protein kinase family
SimilarityBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
SimilarityContains 1 protein kinase domain
SimilarityContains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
SimilarityContains 4 HEAT repeats. {ECO:0000255|PROSITE- ProRule:PRU00103}.
SimilarityContains 6 WD repeats. {ECO:0000255|PROSITE- ProRule:PRU00221}.
Subcellular LocationGolgi apparatus, trans-Golgi network membrane; Lipid-anchor. Endosome membrane; Lipid-anchor.
SubunitComponent of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38
SubunitComponent of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. {ECO:0000269|PubMed:24165940}.