LMPD Database

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LMP007278

UniProt Annotations

Entry Information
Gene Namedolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Protein EntrySTT3_YEAST
UniProt IDP39007
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityDolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
FunctionCatalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. {ECO:0000269|PubMed:12359722}.
InteractionP48439:OST3; NbExp=2; IntAct=EBI-18447, EBI-12680; P32915:SEC61; NbExp=2; IntAct=EBI-18447, EBI-16400; P33767:WBP1; NbExp=3; IntAct=EBI-18447, EBI-12658;
MiscellaneousPresent with 3000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the STT3 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein.
SubunitComponent of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. OST3, OST4 and STT3 probably form a subcomplex. May interact directly with OST3, OST4, OST6, WBP1 and SWP1. Interacts with SEC61. {ECO:0000269|PubMed:10677492, ECO:0000269|PubMed:15831493, ECO:0000269|PubMed:15886282, ECO:0000269|PubMed:9405463, ECO:0000269|PubMed:9435788}.