LMPD Database

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LMP007204

UniProt Annotations

Entry Information
Gene NameWbp1p
Protein EntryOSTB_YEAST
UniProt IDP33767
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityDolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
DomainThe cytoplasmic C-terminal domain contains a functional dilysine-retrieval motif, which is involved in the retrograde Golgi-to-ER transport of the protein.
FunctionEssential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
InteractionP53622:COP1; NbExp=3; IntAct=EBI-12658, EBI-4860; P52870:SBH1; NbExp=2; IntAct=EBI-12658, EBI-16410; P32915:SEC61; NbExp=2; IntAct=EBI-12658, EBI-16400; P35179:SSS1; NbExp=2; IntAct=EBI-12658, EBI-16406; P39007:STT3; NbExp=3; IntAct=EBI-12658, EBI-18447; Q02795:SWP1; NbExp=4; IntAct=EBI-12658, EBI-12666;
MiscellaneousPresent with 14900 molecules/cell in log phase SD medium
MiscellaneousPresent with 14900 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
PathwayProtein modification; protein glycosylation.
SimilarityBelongs to the DDOST 48 kDa subunit family
SimilarityBelongs to the DDOST 48 kDa subunit family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane; Single-pass type I membrane protein.
SubunitComponent of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST2, OST3, OST5, OST6, WBP1 AND SWP1. Interacts with SEC61, SBH1 and SSS1. {ECO:0000269|PubMed:15831493, ECO:0000269|PubMed:15886282, ECO:0000269|PubMed:8175708, ECO:0000269|PubMed:9405463}.