Comment type | Description |
Biophysicochemical Properties | Kinetic parameters: KM=34 mM for p-nitrophenylphosphate {ECO:0000269|PubMed:16442612}; pH dependence: Optimum pH is 7.8. {ECO:0000269|PubMed:16442612}; |
Biophysicochemical Properties | Kinetic parameters: KM=34 mM for p-nitrophenylphosphate ; pH dependence: Optimum pH is 7.8. ; |
Catalytic Activity | [a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate. |
Cofactor | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250}; |
Cofactor | Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ; |
Domain | The TPR repeats mediate protein-protein interactions with substrate proteins, but also autoinhibit PPT1 phosphatase activity. |
Enzyme Regulation | Stimulated by arachidonic acid and other unsaturated fatty acids, and by arachidoyl coenzyme A |
Enzyme Regulation | Stimulated by arachidonic acid and other unsaturated fatty acids, and by arachidoyl coenzyme A. {ECO:0000269|PubMed:12694636}. |
Function | Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery |
Function | Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery. {ECO:0000269|PubMed:16407978}. |
Induction | Expression peaks in early log phase and decreases dramatically during the stationary phase (at protein level) |
Induction | Expression peaks in early log phase and decreases dramatically during the stationary phase (at protein level). {ECO:0000269|PubMed:12694636}. |
Interaction | P15108:HSC82; NbExp=3; IntAct=EBI-13796, EBI-8666; P02829:HSP82; NbExp=8; IntAct=EBI-13796, EBI-8659; |
Miscellaneous | Present with 6990 molecules/cell in log phase SD medium |
Miscellaneous | Present with 6990 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
Sequence Caution | Sequence=CAA61596.1; Type=Frameshift; Positions=381; Evidence= ; |
Sequence Caution | Sequence=CAA61596.1; Type=Frameshift; Positions=381; Evidence={ECO:0000305}; |
Similarity | Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily |
Similarity | Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily. {ECO:0000305}. |
Similarity | Contains 3 TPR repeats. {ECO:0000255|PROSITE- ProRule:PRU00339}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:12694636, ECO:0000269|PubMed:14562095}. |
Subunit | Interacts (via TPR repeats) with HSP82 (via C-terminal MEEVD pentapeptide) |
Subunit | Interacts (via TPR repeats) with HSP82 (via C-terminal MEEVD pentapeptide). {ECO:0000269|PubMed:16407978}. |