LMPD Database

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LMP006878

UniProt Annotations

Entry Information
Gene Nametype 2A-related serine/threonine-protein phosphatase SIT4
Protein EntryPP11_YEAST
UniProt IDP20604
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic Activity[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate.
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CofactorName=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ;
Developmental StageFunctions in the late cell cycle G1 phase for progression into the S phase, possibly associated in two separate complexes with the phosphorylated forms of p155 and p190, two high MW proteins.
FunctionInvolved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.
InteractionP40454:RRD1; NbExp=4; IntAct=EBI-13707, EBI-25278; P43612:SAP155; NbExp=8; IntAct=EBI-13707, EBI-16370; P40856:SAP185; NbExp=8; IntAct=EBI-13707, EBI-16384; P36123:SAP190; NbExp=4; IntAct=EBI-13707, EBI-16392; Q04372:TAP42; NbExp=6; IntAct=EBI-13707, EBI-18926;
MiscellaneousPresent with 3970 molecules/cell in log phase SD medium
MiscellaneousPresent with 3970 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
SimilarityBelongs to the PPP phosphatase family. PP-6 (PP-V) subfamily
SimilarityBelongs to the PPP phosphatase family. PP-6 (PP-V) subfamily. {ECO:0000305}.
Subcellular LocationCytoplasm.
SubunitInactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD1-TAP42. {ECO:0000269|PubMed:12820961, ECO:0000269|PubMed:14551259, ECO:0000269|PubMed:15447631, ECO:0000269|PubMed:15689491}.