LMPD Database

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LMP006865

UniProt Annotations

Entry Information
Gene NameBud32p
Protein EntryBUD32_YEAST
UniProt IDP53323
SpeciesYeast (S288c)
Comments
Comment typeDescription
Catalytic ActivityATP + a protein = ADP + a phosphoprotein.
FunctionComponent of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection. {ECO:0000269|PubMed:16564010, ECO:0000269|PubMed:16874308, ECO:0000269|PubMed:21183954, ECO:0000269|PubMed:21459853, ECO:0000269|PubMed:23258706, ECO:0000269|PubMed:23620299}.
InteractionQ03705:CGI121; NbExp=8; IntAct=EBI-3809, EBI-912262; P46984:GON7; NbExp=6; IntAct=EBI-3809, EBI-26178; P32642:GRX4; NbExp=10; IntAct=EBI-3809, EBI-22211; P36132:KAE1; NbExp=20; IntAct=EBI-3809, EBI-26411;
MiscellaneousPresent with 3020 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
MiscellaneousThis protein is considered an atypical serine/threonine kinase, because it lacks the conventional structural elements necessary for the substrate recognition as well as a lysine residue that in all other serine/threonine kinases participates in the catalytic event (PubMed:12023889). BUD32 has protein kinase activity in vitro, but in the context of the EKC/KEOPS complex, the catalytic subunit KAE1 switches the activity of BUD32 from kinase into ATPase (By similarity). {ECO:0000250, ECO:0000305|PubMed:12023889}.
SimilarityBelongs to the protein kinase superfamily. BUD32 family. {ECO:0000305}.
SimilarityContains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:14562095}. Nucleus {ECO:0000269|PubMed:14562095}. Chromosome, telomere {ECO:0000269|PubMed:14562095}.
SubunitComponent of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes. {ECO:0000269|PubMed:16564010, ECO:0000269|PubMed:16874308}.