LMPD Database

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LMP006735

UniProt Annotations

Entry Information
Gene NameN-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Protein EntryASAH2_HUMAN
UniProt IDQ9NR71
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NR71-1; Sequence=Displayed; Name=2; IsoId=Q9NR71-2; Sequence=VSP_019928; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=71.4 uM for octanoyl-sphingosine ; KM=66 uM for palmitoyl-sphingosine {ECO
Catalytic ActivityN-acylsphingosine + H(2)O = a carboxylate + sphingosine. {ECO
Enzyme RegulationInhibited by dithiothreitol (DTT), 2- mercaptoethanol, Zn(2+), Cu(2+) and Fe(2+). Enhanced by Na(+) and Ca(2+), and at lower level Mg(2+) and Mn(2+).
FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. {ECO
PtmN-glycosylated. Required for enzyme activity (By similarity).
PtmO-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
PtmPhosphorylated. May prevent ubiquitination and subsequent degradation (By similarity).
PtmUbiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid (By similarity).
Sequence CautionSequence=AAL06061.1; Type=Erroneous initiation; Evidence= ; Sequence=CAI15870.1; Type=Erroneous initiation; Evidence= ;
SimilarityBelongs to the neutral ceramidase family.
Subcellular LocationCell membrane ; Single-pass type II membrane protein . Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo.
Tissue SpecificityPrimarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. {ECO