LMPD Database

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LMP006573

UniProt Annotations

Entry Information
Gene Namesterol regulatory element binding factor 2
Protein EntryQ3U1N2_MOUSE
UniProt IDQ3U1N2
SpeciesMouse
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1 ; IsoId=Q3U1N2-1; Sequence=Displayed; Name=2 ; IsoId=Q3U1N2-2; Sequence=VSP_052658, VSP_052659; Note=No experimental confirmation available. ;
Disruption PhenotypeDeath around embryonic day 7-8.
FunctionTranscriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway. Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
InteractionQ9JM73:Srf; NbExp=3; IntAct=EBI-645275, EBI-493266;
MiscellaneousMice overexpressing processed Srebf2 show preferential activation of cholesterol over fatty acid synthesis in liver and adipose tissue.
PtmAt low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7 (By similarity).
PtmPhosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
SimilarityContains 1 bHLH (basic helix-loop-helix) domain.
Subcellular LocationEndoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein . Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Subcellular LocationProcessed sterol regulatory element-binding protein 2: Nucleus {ECO:0000250|UniProtKB:Q12772, ECO:0000255|PROSITE-ProRule:PRU00981}.
SubunitForms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein (By similarity). Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139 (By similarity).