| Comment type | Description |
|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=3; Name=1; Synonyms=Alpha; IsoId=Q6NYC1-1; Sequence=Displayed; Name=2; Synonyms=Beta; IsoId=Q6NYC1-2; Sequence=VSP_014022, VSP_014023; Name=3; IsoId=Q6NYC1-3; Sequence=VSP_014023; |
| Biophysicochemical Properties | Kinetic parameters: KM=39 uM for 2-oxoglutarate ; |
| Caution | Was initially thought to constitute the phosphatidylserine receptor, a receptor that mediates recognition of phosphatidylserine, a specific marker only present at the surface of apoptotic cells. Phosphatidylserine receptor probably participates in apoptotic cell phagocytosis. This protein was identified using phage display expressing mAb 217, an antibody that specifically recognizes phosphatidylserine receptor. However, its nuclear localization and the fact that mAb 217 antibody still recognizes the phosphatidylserine receptor in mice lacking JMJD6, strongly suggest that it does not constitute the receptor for phosphatidylserine and is not involved in apoptotic cell removal. |
| Cofactor | Name=Fe(2+); Xref=ChEBI |
| Domain | The nuclear localization signal motifs are necessary and sufficient to target it into the nucleus. |
| Function | Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. {ECO |
| Induction | Up-regulated upon cytokine treatment, but not upon TNF treatment. |
| Interaction | Self; NbExp=3; IntAct=EBI-8464037, EBI-8464037; O60885:BRD4; NbExp=10; IntAct=EBI-8464037, EBI-723869; P50750:CDK9; NbExp=5; IntAct=EBI-8464037, EBI-1383449; Q8NAV1:PRPF38A; NbExp=2; IntAct=EBI-8464037, EBI-715374; Q01081:U2AF1; NbExp=2; IntAct=EBI-8464037, EBI-632461; Q9NP64:ZCCHC17; NbExp=2; IntAct=EBI-8464037, EBI-746345; |
| Sequence Caution | Sequence=AAH47003.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ; Sequence=BAA25511.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ; |
| Similarity | Belongs to the JMJD6 family. |
| Similarity | Contains 1 JmjC domain. {ECO |
| Subcellular Location | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle. |
| Subunit | Interacts with LUC7L2, LUC7L3 and U2AF2/U2AF65. Interacts with BRD4. {ECO |
| Tissue Specificity | Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells. {ECO |