LMPD Database

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LMP005459

UniProt Annotations

Entry Information
Gene Namealdo-keto reductase family 1, member C21
Protein EntryAK1CL_MOUSE
UniProt IDQ91WR5
SpeciesMouse
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=1.8 uM for NADP {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=19 uM for androstenedione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.5 uM for 5-beta-pregnane-3-alpha,20-alpha-diol {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.5 uM for 5-beta-pregnane-3,20-dione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.6 uM for epitestosterone {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; KM=0.3 uM for androst-4-ene-3,17-dione {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338}; pH dependence: Optimum pH is 10.0. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338};
Catalytic ActivityAndrosterone + NAD(P)(+) = 5-alpha-androstane- 3,17-dione + NAD(P)H. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}.
Enzyme RegulationInhibited by high concentrations of substrate. {ECO:0000269|PubMed:15577209}.
FunctionNADP-dependent 17-alpha-hydroxysteroid dehydrogenase that converts 5-alpha-androstane-3,17-dione into androsterone. Has lower 3-alpha-hydroxysteroid dehydrogenase activity. Has broad substrate specificity and acts on various 17-alpha- hydroxysteroids, 17-ketosteroids, 3-alpha hydroxysteroids and 3- ketosteroids. Reduction of keto groups is strictly stereoselective. Reduction of 17-ketosteroids yields only 17- alpha-hydroxysteroids. Likewise, reduction of 3-ketosteroids yields only 3-alpha-hydroxysteroids. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:16018803, ECO:0000269|PubMed:17034817, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:20124700}.
SimilarityBelongs to the aldo/keto reductase family. {ECO:0000305}.
Subcellular LocationCytoplasm {ECO:0000250}.
SubunitMonomer. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:17034817, ECO:0000269|PubMed:17442338, ECO:0000269|PubMed:17909281, ECO:0000269|PubMed:19237748}.
Tissue SpecificityDetected in kidney and brain. {ECO:0000269|PubMed:15577209, ECO:0000269|PubMed:16018803}.