LMPD Database

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LMP004886

UniProt Annotations

Entry Information
Gene NameRab geranylgeranyl transferase, b subunit
Protein EntryQ3TVF4_MOUSE
UniProt IDQ3TVF4
SpeciesMouse
Comments
Comment typeDescription
Catalytic ActivityGeranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate. {ECO:0000269|PubMed:21520375}.
CofactorName=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:21520375}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:21520375};
Developmental StageSpecific expression was elevated in mid- gestation stages, particularly developing liver and spinal cord. {ECO:0000269|PubMed:9031634}.
Enzyme RegulationThe enzymatic reaction requires the aid of a Rab escort protein (also called component A), such as CHM.
FunctionCatalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A. {ECO:0000269|PubMed:21520375}.
InductionIncreased dramatically by cycloheximide (CHX) treatment within a short time (as early as 2 hours). Actinomycin D was used to determine the half-life, CHX treatment resulted in a dramatic increase of the half-life from 8 hours to greater than 12 hours. {ECO:0000269|PubMed:9031634}.
SimilarityBelongs to the protein prenyltransferase subunit beta family. {ECO:0000305}.
SimilarityContains 6 PFTB repeats. {ECO:0000305}.
SubunitHeterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding. The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp). The CHM:RGGT:Rab complex is destabilized by GGpp. Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity (By similarity). {ECO:0000250}.
Tissue SpecificityUbiquitous. Detected in all the major organs in adult animals. {ECO:0000269|PubMed:9031634}.