LMPD Database

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LMP003575

UniProt Annotations

Entry Information
Gene Namephosphoprotein associated with glycosphingolipid microdomains 1
Protein EntryPHAG1_MOUSE
UniProt IDQ3U1F9
SpeciesMouse
Comments
Comment typeDescription
Disruption PhenotypeMice are viable and do not show any developmental defect up to 20 months of age. They have normal T- cell development and normal T- and B-cell responses. {ECO:0000269|PubMed:16166631}.
FunctionNegatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. {ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}.
PtmPalmitoylated. {ECO:0000269|PubMed:12626544}.
PtmPhosphorylated by FYN on Tyr-314 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:14967142, ECO:0000269|PubMed:17947660, ECO:0000269|PubMed:19144319}.
Subcellular LocationCell membrane {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}; Single-pass type III membrane protein {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14645715, ECO:0000269|PubMed:16166631}. Note=Present in lipid rafts.
SubunitWhen phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. {ECO:0000269|PubMed:11777944, ECO:0000269|PubMed:12218089, ECO:0000269|PubMed:12612075, ECO:0000269|PubMed:14967142}.
Tissue SpecificityPresent in T-cells (at protein level). {ECO:0000269|PubMed:16166631}.