LMPD Database

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LMP002785

UniProt Annotations

Entry Information
Gene Namelipoic acid synthetase
Protein EntryLIAS_MOUSE
UniProt IDQ99M04
SpeciesMouse
Comments
Comment typeDescription
Catalytic ActivityProtein N(6)-(octanoyl)lysine + 2 sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)- (lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'- deoxyadenosine.
CofactorName=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255|HAMAP-Rule:MF_03123}; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. {ECO:0000255|HAMAP-Rule:MF_03123};
FunctionCatalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. {ECO:0000305}.
PathwayProtein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. {ECO:0000255|HAMAP-Rule:MF_03123}.
SimilarityBelongs to the radical SAM superfamily. Lipoyl synthase family. {ECO:0000255|HAMAP-Rule:MF_03123}.
Subcellular LocationMitochondrion {ECO:0000255|HAMAP- Rule:MF_03123, ECO:0000269|PubMed:11389890}.
Tissue SpecificityExpressed predominantly in heart, testis, and liver. {ECO:0000269|PubMed:11389890}.