LMPD Database

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LMP002075

UniProt Annotations

Entry Information
Gene Namephospholipase C, delta 4
Protein EntryPLCD4_MOUSE
UniProt IDQ8K3R3
SpeciesMouse
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q8K3R3-1; Sequence=Displayed; Name=2; IsoId=Q8K3R3-2; Sequence=VSP_028503; Note=No experimental confirmation available.; Name=3; IsoId=Q8K3R3-3; Sequence=VSP_028503, VSP_028506; Note=No experimental confirmation available.; Name=4; IsoId=Q8K3R3-4; Sequence=VSP_028504, VSP_028505; Note=No experimental confirmation available.;
Catalytic Activity1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Note=Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain. {ECO:0000250};
Disruption PhenotypeMice are either sterile or produce few small litters. In these mice, fewer eggs become activated and the Ca(2+) transients associated with fertilization are absent or delayed. Sperm are unable to initiate the acrosome reaction. {ECO:0000269|PubMed:11340203}.
DomainThe C2 domain mediates pre-localization to the membrane prior to Ca(2+) import and non-selective Ca(2+)-mediated targeting to various cellular membranes. {ECO:0000250}.
DomainThe PDZ-binding motif mediates the interaction with GRIP1.
DomainThe PH domain is not a critical determinant of the membrane localization. {ECO:0000250}.
FunctionHydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida- induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. {ECO:0000269|PubMed:11340203, ECO:0000269|PubMed:12695499, ECO:0000269|PubMed:16998201}.
InductionBy treatment with growth factors such as bradykinin, lysophosphatidic acid, and Ca(2+) ionophore in addition to serum. {ECO:0000269|PubMed:10601847}.
SimilarityContains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
SimilarityContains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}.
SimilarityContains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}.
SimilarityContains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}.
SimilarityContains 3 EF-hand domains. {ECO:0000255|PROSITE- ProRule:PRU00448}.
Subcellular LocationMembrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}. Note=Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum. {ECO:0000250}.
SubunitInteracts with GRIP1. {ECO:0000269|PubMed:16272139}.