LMPD Database

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LMP002007

UniProt Annotations

Entry Information
Gene Namecytochrome P450, family 4, subfamily F, polypeptide 2
Protein EntryCP4F2_HUMAN
UniProt IDP78329
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P78329-1; Sequence=Displayed; Name=2; IsoId=P78329-2; Sequence=VSP_055578, VSP_055579, VSP_055580; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=74.8 uM for leukotriene-B(4) ; KM=1.7 uM for menaquinone-4 (MK-4) ; Vmax=2.42 nmol/min/mg enzyme ; Note=kcat is 0.067 min(-1) with menaquinone-4 (MK-4) as substrate ;
Catalytic Activity(5Z,8Z,11Z,14Z)-icosatetraenoate + NADPH + O(2) = (5Z,8Z,11Z,14Z)-20-hydroxyicosa-5,8,11,14-tetraenoate + NADP(+) + H(2)O.
Catalytic Activity(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa- 6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)- 5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.
Catalytic ActivityPhylloquinone + NADPH + O(2) = omega- hydroxyphylloquinone + NADP(+) + H(2)O.
CofactorName=heme; Xref=ChEBI
DiseaseCoumarin resistance (CMRES) [MIM
FunctionOmega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B(4) omega-hydroxylase by mediating conversion of leukotriene-B(4) (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene- B(4) (20-OH LTB4) (PubMed:8026587, PubMed:9799565). {ECO
InteractionP16333:NCK1; NbExp=2; IntAct=EBI-1752413, EBI-389883;
PathwayCofactor degradation; phylloquinone degradation.
SimilarityBelongs to the cytochrome P450 family.
Subcellular LocationMicrosome membrane {ECO
Tissue SpecificityLiver. Also present in kidney: specifically expressed in the S2 and S3 segments of proximal tubules in cortex and outer medulla (PubMed:10660572). {ECO
Web ResourceName=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cyp4f2/";