LMPD Database

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LMP001538

UniProt Annotations

Entry Information
Gene Namehematopoietic prostaglandin D synthase
Protein EntryHPGDS_HUMAN
UniProt IDO60760
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=8 mM for glutathione for the glutathione-conjugating activity {ECO
Catalytic Activity(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15- hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy- 11-oxoprosta-5,13-dienoate.
Catalytic ActivityRX + glutathione = HX + R-S-glutathione.
CofactorName=glutathione; Xref=ChEBI
Developmental StageHighest levels in immature megakaryocytic cells. Disappears after final differentiation to platelets.
Enzyme RegulationProstaglandin PGD2 synthesis is stimulated by calcium and magnesium ions. One calcium or magnesium ion is bound between the subunits of the homodimer. The interactions with the protein are for the most part mediated via water molecules. Magnesium increases the affinity for glutathione, while calcium has no effect on the affinity for glutathione.
FunctionBifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide. {ECO
InductionBy 12-O-tetradecanoylphorbol-13-acetate (TPA).
SimilarityBelongs to the GST superfamily. Sigma family.
SimilarityContains 1 GST C-terminal domain.
SimilarityContains 1 GST N-terminal domain.
Subcellular LocationCytoplasm .
SubunitHomodimer. {ECO
Tissue SpecificityExpressed in a number of megakaryocytic cell lines but not in platelets. Highly expressed in adipose tissue, macrophages and placenta. Also expressed at lower levels in lung, heart, lymph nodes, appendix, bone marrow and fetal liver. {ECO