LMPD Database

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LMP000670

UniProt Annotations

Entry Information
Gene Namecytochrome P450, family 2, subfamily a, polypeptide 4
Protein EntryCP2A4_MOUSE
UniProt IDP15392
SpeciesMouse
Comments
Comment typeDescription
Catalytic ActivityRH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.
CofactorName=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
FunctionHighly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol.
MiscellaneousThere are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7- hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.
SimilarityBelongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue SpecificityKidney and lung. Expressed in liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP. {ECO:0000269|PubMed:10490589}.