LMPD Database

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LMP000423

UniProt Annotations

Entry Information
Gene Namebile acid CoA:amino acid N-acyltransferase
Protein EntryBAAT_HUMAN
UniProt IDQ14032
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=1.1 mM for taurine toward choloyl-CoA ; KM=2.2 mM for 2-fluoro-beta-alanine toward choloyl-CoA ; KM=5.8 mM for glycine toward choloyl-CoA ; KM=19.3 uM for arachidoyl-CoA ; Vmax=0.33 umol/min/mg enzyme with taurine as substrate for acyltransferase activity ; Vmax=0.19 umol/min/mg enzyme with 2-fluoro-beta-alanine as substrate for acyltransferase activity ; Vmax=0.77 umol/min/mg enzyme with glycine as substrate for acyltransferase activity ; Vmax=223 nmol/min/mg enzyme with arachidoyl-CoA as substrate for acyl-CoA thioesterase activity ;
Catalytic ActivityCholoyl-CoA + glycine = CoA + glycocholate.
Catalytic ActivityPalmitoyl-CoA + H(2)O = CoA + palmitate.
DiseaseFamilial hypercholanemia (FHCA) [MIM
FunctionInvolved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
MiscellaneousIn human, more than 95% of the biliary bile acids are N-acyl amidates with glycine and taurine. In other mammalian species large differences are observed in the relative amounts of taurine- and glycine-conjugated bile acids formed in bile.
SimilarityBelongs to the C/M/P thioester hydrolase family.
Subcellular LocationCytoplasm .
SubunitMonomer.
Tissue SpecificityExpressed in liver, gallbladder mucosa and pancreas. {ECO