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Lipid Standards: Eicosanoids

LC Retention Times

LC retention time and MS/MS data were obtained using the conditions outlined in the protocols below
Eicos LC Protocol[1]| Eicos LC Protocol_V2[2]| MS/MS Protocol
lm_id name Systematic Name MS/MS R.T.(min)[1] R.T.(min)[2]
LMFA03010139 tetranor-PGFM 9S,11R-dihydroxy-15-oxo-2,3,4,5-tetranor-prostan-1,20-dioic acid 329([M-H]-) 2.55 2.35
LMFA03010029 20-hydroxy-PGF2a 9S,11S,15S,20-tetrahydroxy-5Z,13E-prostadienoic acid 369([M-H]-) 2.91 2.8
LMFA03010014 20-hydroxy-PGE2 9-oxo-11R,15S,20-trihydroxy-5Z,13E-prostadienoic acid 367([M-H]-) 2.94 2.91
LMFA03010024 19R-hydroxy-PGE2 9-oxo-11R,15S,19R-trihydroxy-5Z,13E-prostadienoic acid 367([M-H]-) 2.95 2.97
LMFA03010001 6-keto-PGF1α 6-oxo-9S,11R,15S-trihydroxy-13E-prostenoic acid 369([M-H]-) 3.4 4.71
LMFA03010012 6keto-PGE1 6,9-dioxo-11R,15S-dihydroxy-13E-prostenoic acid 367([M-H]-) 3.66 5.19
LMFA03010025 PGF2β 9R,11R,15S-trihydroxy-5Z,13E-prostadienoic acid 353([M-H]-) 4.09 6.59
LMFA03030002 TXB2 9S,11,15S-trihydroxy-thromboxa-5Z,13E-dien-1-oic acid 369([M-H]-) 4.2 6.61
LMFA03010013 6,15-diketo-13,14-dihydro-PGF1α 6,15-dioxo-9S,11R-dihydroxyprostanoic acid 369([M-H]-) 4.24 6.13
LMFA03010036 11β-PGF2α 9S,11S,15S-trihydroxy-5Z,13E-prostadienoic acid 353([M-H]-) 4.34 6.58
LMFA03010002 PGF2α 9S,11R,15S-trihydroxy-5Z,13E-prostadienoic acid 353([M-H]-) 4.72 7.35
LMFA03010011 2,3-dinor 11β-PGF2a 9S,11S,15S-trihydroxy-2,3-dinor-5Z,13E-prostadienoic acid 325([M-H]-) 3.48 4.86
LMFA03010003 PGE2 9-oxo-11R,15S-dihydroxy-5Z,13E-prostadienoic acid 351([M-H]-) 5.19 7.54
LMFA03010023 PGK2 9,11-dioxo-15S-hydroxy-5Z,13E-prostadienoic acid 349([M-H]-) 5.42 8.3
LMFA03010026 15-keto-PGF2α 9S,11R-dihydroxy-15-oxo-5Z,13E-prostadienoic acid 351([M-H]-) 5.47 7.89
LMFA03010004 PGD2 9S,15S-dihydroxy-11-oxo-5Z,13E-prostadienoic acid 351([M-H]-) 5.69 7.89
LMFA03010030 15-keto-PGE2 9,15-dioxo-11R-hydroxy-5Z,13E-prostadienoic acid 349([M-H]-) 6.03 8.02
LMFA03010027 13,14-dihydro-15-keto-PGF2α 9S,11S-dihydroxy-15-oxo-5Z-prostenoic acid 353([M-H]-) 6.57 8.76
LMFA03040001 5S,6R-LipoxinA4 5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid 351([M-H]-) 6.78 8.58
LMFA03010031 13,14-dihydro-15-keto-PGE2 9,15-dioxo-11R-hydroxy-5Z-prostenoic acid 351([M-H]-) 6.81 8.6
LMFA03010010 PGH2 9S,11R-epidioxy-15S-hydroxy-5Z,13E-prostadienoic acid 351([M-H]-) 7.55 7.53
LMFA03010022 13,14-dihydro-15-keto-PGD2 11,15-dioxo-9S-hydroxy-5Z-prostenoic acid 351([M-H]-) 7.75 9.08
LMFA03010035 PGA2 9-oxo-15S-hydroxy-5Z,10Z,13E-prostatrienoic acid 333([M-H]-) 8.22 9.75
LMFA03010019 PGJ2 11-oxo-15S-hydroxy-5Z,9,13E-prostatrienoic acid 333([M-H]-) 8.39 9.95
LMFA03010018 PGB2 15S-hydroxy-9-oxo-5Z,8(12),13E-prostatrienoic acid 333([M-H]-) 8.42 9.82
LMFA03010033 13,14-dihydro-15 keto-PGA2 9,15-dioxo-5Z,10-prostadienoic acid 333([M-H]-) 9.12 10.32
LMFA03060010 5S,15S-DiHETE 5S,15S-dihydroxy-6E,8Z,11Z,13E-eicosatetraenoic acid 335([M-H]-) 9.2 11.03
LMFA03050010 (+/-)14,15-DiHETrE 14,15-dihydroxy-5Z,8Z,11Z-eicosatrienoic acid 337([M-H]-) 9.67 12.7
LMFA03050008 (+/-)11,12-DiHETrE 11,12-dihydroxy-5Z,8Z,14Z-eicosatrienoic acid 337([M-H]-) 9.84 13.01
LMFA03010021 15-deoxy-δ-12,14-PGJ2 11-oxo-5Z,9,12E,14E-prostatetraenoic acid 315([M-H]-) 9.98 13.01
LMFA03050006 (+/-)8,9-DiHETrE 8,9-dihydroxy-5Z,11Z,14Z-eicosatrienoic acid 337([M-H]-) 9.99 13.4
LMFA03060018 5S,6S-DiHETE 5S,6S-dihydroxy-7E,9E,11Z,14Z-eicosatetraenoic acid 335([M-H]-) 10.2 13.8
LMFA03050004 (+/-)5,6-DiHETrE 5,6-dihydroxy-8Z,11Z,14Z-eicosatrienoic acid 337([M-H]-) 10.25 14.04
LMFA03020003 LTC4 5S-hydroxy,6R-(S-glutathionyl),7E,9E,11Z,14Z-eicosatetraenoic acid 624([M-H]-) 10.25 12.99
LMFA03060001 15S-HETE 15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid 319([M-H]-) 10.45 14.33
LMFA03060014 15S-HpETE 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid 335([M-H]-) 10.5 14.48
LMFA03060003 11S-HETE 11S-hydroxy-5Z,8Z,12E,14Z-eicosatetraenoic acid 319([M-H]-) 10.62 14.83
LMFA03060019 12-oxo-ETE 12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid 317([M-H]-) 10.67 14.59
LMFA03060008 12R-HETE 12R-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid 319([M-H]-) 10.7 14.77
LMFA03060007 12S-HETE 12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid 319([M-H]-) 10.7 14.8
LMFA03060006 8S-HETE 8S-hydroxy-5Z,9E,11Z,14Z-eicosatetraenoic acid 319([M-H]-) 10.76 14.86
LMFA03060002 5S-HETE 5S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 319([M-H]-) 11.06 15.36
LMFA03060012 5S-HpETE 5S-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 335([M-H]-) 11.08 15.42
LMFA03060011 5-Oxo-ETE 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid 317([M-H]-) 11.27 15.51
LMFA03080004 (+/-)11,12-EpETrE 11,12-epoxy-5Z,8Z,14Z-eicosatrienoic acid 319([M-H]-) 11.36 15.83
LMFA03080003 (+/-)8,9-EpETrE 8,9-epoxy-5Z,11Z,14Z-eicosatrienoic acid 319([M-H]-) 11.5 16.05
LMFA03080002 (+/-)5,6-EpETrE 5,6-epoxy-8Z,11Z,14Z-eicosatrienoic acid 319([M-H]-) 11.7 16.3
LMFA01030001 Arachidonic acid 5Z,8Z,11Z,14Z-eicosatetraenoic acid 303([M-H]-) 14.37 18.97
Total of 48 Records