Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin

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PA| PC| PE| PG| PI| PS| DNA| TNF

Details/Graph
LM_ID Name Kdo+Comp
Kdo
Comp
Ctrl
0 hrs
Kdo+Comp
Kdo
Comp
Ctrl
0.5 hrs
Kdo+Comp
Kdo
Comp
Ctrl
1 hr
Kdo+Comp
Kdo
Comp
Ctrl
2 hrs
Kdo+Comp
Kdo
Comp
Ctrl
4 hrs
Kdo+Comp
Kdo
Comp
Ctrl
8 hrs
Kdo+Comp
Kdo
Comp
Ctrl
12 hrs
Kdo+Comp
Kdo
Comp
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24 hrs
Units
. . . .
     (BCH081021) PS(32:0) -
-
-
6.32
1.09
0.01
6.17
7.92
3.97
1.43
1.55
1.73
1.83
1.26
0.46
0.55
1.39
1.91
1.64
1.58
1.4
1.63
2.19
1.91
1.79
1.76
1.48
1.34
18
3.22
4.6
1.67
pmol/ug DNA
     (BCH081028) PS(32:0) -
-
-
1.6
1.57
1.28
0.83
0.79
0.82
5.33
6.49
7.22
8.61
7.77
6.94
6.4
9.12
10.5
9.58
8.68
1.86
0.06
0.05
0.14
12.7
0.11
1.63
2.8
1.44
2.23
1.09
1.3
pmol/ug DNA
     (BCH081209) PS(32:0) -
-
-
18.4
14.5
15.9
16
15.1
16.5
15.7
12.2
15.7
19.1
15
16.5
17
17.9
18.7
16.4
14.1
18.4
17.3
14.8
17.5
20.7
24
17.1
23.1
20.2
24.6
19.2
22.2
pmol/ug DNA
     (BCH081021) PS(32:1) -
-
-
1.86
3.41
1.64
0.32
2.16
3.61
2.37
2.88
6.71
2.81
3.68
2.31
2.14
3.22
6.31
2.65
2.08
1.73
1.97
2.71
2.58
2.75
1.89
1.75
1.64
2.06
4.18
1.08
2.47
pmol/ug DNA
     (BCH081028) PS(32:1) -
-
-
4.83
3.19
2.81
2.26
2.11
2.58
2.57
3.12
1.28
2.26
1.72
1.37
2.2
1.85
2.2
2.54
2.4
2.1
1.59
2.19
2.43
3.93
2.56
2.21
4.7
1.49
2.3
2.18
3.05
pmol/ug DNA
     (BCH081209) PS(32:1) -
-
-
3.79
3.7
4.39
3.39
3.23
4.76
4.17
3.7
4.29
5.25
4.25
4.47
4.79
4.99
5.08
4.96
4.09
5.78
4.97
4.81
5.03
5.95
7
5.48
7.05
7.22
8.33
9.95
10
pmol/ug DNA
     (BCH081021) PS(34:0) -
-
-
18.8
20.9
27.8
18.8
-
22.5
27.5
27.6
27.3
17.6
21.5
23.1
25.2
26
24.4
24.7
23.8
29
26.4
18.2
17.5
26
24.6
26
27.4
29.5
33.6
11.8
27.7
pmol/ug DNA
     (BCH081028) PS(34:0) -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(34:0) -
-
-
28.6
21.2
23.2
23
22.3
24.1
23.4
18.2
24.1
26.9
21.8
23.5
23.6
24.6
25.1
23.2
21.2
24.4
21.8
21.3
24.3
25
31.1
22.3
31.5
27.5
32.3
23.4
25.8
pmol/ug DNA
     (BCH081021) PS(34:1) -
-
-
25.1
31.6
44
25.6
26.9
31.2
39.5
40.6
39.8
27.5
30.6
35.8
35.8
38
36.8
38.4
36.2
44.3
39.5
28.5
26.3
46.6
35.9
41.8
42.7
58.6
59.1
23
51.1
pmol/ug DNA
     (BCH081028) PS(34:1) -
-
-
26.7
42.5
25.5
25.4
28.1
24.2
28.9
32.1
29.9
40.6
36.3
31
29.9
39.9
46.8
44.3
42.2
57.2
36.3
50.1
48.6
50.6
52.8
40.7
42.8
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(34:1) -
-
-
42
28.3
33.4
33
31.6
35.3
32.4
28.1
33.2
39
30.4
33.4
35.9
35.6
37.2
36
30.3
38.5
33.9
34.3
37
37.4
44.9
36.7
49.4
46.9
52.9
54.3
53.5
pmol/ug DNA
     (BCH081021) PS(34:2) -
-
-
8.47
10.3
12.8
9.17
11.2
11.5
15
13.8
13.4
10.1
10.1
12.3
14
15.7
12.4
13.7
13
15.3
13.9
12
10.1
16.9
13.2
13.9
13.5
20.1
22.9
7.11
17
pmol/ug DNA
     (BCH081028) PS(34:2) -
-
-
1.03
5.9
0.73
0.8
0.71
2.65
3.57
4.02
3.92
4.72
4.39
3.77
3.53
5.85
5.82
5.69
4.85
0.2
6.02
0.33
0.46
6.65
7.43
-
2.42
1.12
1.11
0.92
1.56
pmol/ug DNA
     (BCH081209) PS(34:2) -
-
-
15.5
10.9
12.9
12.6
12.4
13.2
12.8
9.96
12.8
15.2
12.2
13.3
13.7
13.1
13.9
13.7
11.6
14.5
13.6
13.3
14.4
14.5
16.9
13.5
18
16.3
18.8
20.3
19.7
pmol/ug DNA
     (BCH081021) PS(34:3) -
-
-
1.84
1.9
1.68
1.07
5.16
7.74
2.63
5.16
4.31
3.03
3.22
2.33
1.8
1.86
3.81
3.23
3.03
2.7
4.52
3.76
2.84
4.07
3.06
2.74
2.45
2.73
3.26
1.57
2.9
pmol/ug DNA
     (BCH081028) PS(34:3) -
-
-
3.47
2.74
2.41
1.26
1.41
1.66
1.47
1.54
1.39
2.12
1.33
1.13
1.29
1.66
2.54
2.01
1.98
2.52
1.38
1.73
2.65
4.86
2.45
2.46
4.49
2.3
3.31
2.05
2.79
pmol/ug DNA
     (BCH081209) PS(34:3) -
-
-
6.02
3.56
4.1
4.07
3.9
4.54
4.42
3.62
4.24
5.26
3.98
0.95
4.61
4.7
4.5
4.34
4.27
4.73
3.88
4.42
4.28
4.48
5.16
3.9
5.61
5.33
6.35
5.33
4.26
pmol/ug DNA
     (BCH081021) PS(36:0) -
-
-
10.7
8.19
6.97
20.3
69.8
0.65
8.93
9.51
13.3
10.6
8.69
8.77
11.4
8.65
9.11
11.2
-
9.11
9.94
9.88
4.79
6.96
-
9.52
7.6
12
-
8.16
7.39
pmol/ug DNA
     (BCH081028) PS(36:0) -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(36:0) -
-
-
8.36
13.1
13.9
14.7
15.8
17.7
17.1
14.7
18.9
18.1
14.9
16.4
16.9
16.7
16.4
17.2
15.6
18
15
15.1
16.3
10.9
20.4
18.5
24.4
24
29.6
25
12.6
pmol/ug DNA
     (BCH081021) PS(36:1) -
-
-
71.9
62.6
92.5
-
-
60.9
82.7
86.8
78.8
61.8
72.1
80.2
83.1
74.3
70.3
77.2
73.3
92.9
75.5
55.4
48.9
86.2
76.5
95.6
99.2
133
137
56.8
126
pmol/ug DNA
     (BCH081028) PS(36:1) -
-
-
87.8
125
87.4
87.7
93.5
70.8
89.5
98.7
81.4
107
104
86.2
86.3
85.4
107
114
113
122
83.7
124
118
99.9
119
95.9
81.9
7.8
6.62
6.25
5.72
pmol/ug DNA
     (BCH081209) PS(36:1) -
-
-
128
80.5
95.8
96
94.8
108
98.4
84.4
98.7
108
89.3
98.5
101
102
99.9
103
93
106
90.9
104
104
95.3
119
104
139
135
156
148
138
pmol/ug DNA
     (BCH081021) PS(36:2) -
-
-
81
91.9
129
84
101
84.3
113
117
114
85.7
98.2
105
114
101
98.8
109
102
126
112
74.7
68.5
123
105
122
125
167
161
67.7
148
pmol/ug DNA
     (BCH081028) PS(36:2) -
-
-
0.9
63.8
2.25
0.96
0.73
36.9
46.5
49.1
42.2
57.6
51.1
43.5
43.7
49
59.5
59.8
60.2
0.21
0.32
0.35
0.62
56.3
0.3
2.09
2.43
0.53
1.88
1.42
1.57
pmol/ug DNA
     (BCH081209) PS(36:2) -
-
-
152
94.8
114
110
114
122
112
95.7
114
127
104
111
120
117
118
118
102
120
103
119
120
110
134
115
155
142
160
154
146
pmol/ug DNA
     (BCH081021) PS(36:4) -
-
-
6.64
7.64
12.3
7.91
6
6.7
10.4
10.2
-
6.61
7.85
7.4
8.42
10.5
8.46
8.39
8.54
9.65
9.65
6.05
6.29
9.23
-
9.41
10.7
15.1
-
6.6
11.7
pmol/ug DNA
     (BCH081028) PS(36:4) -
-
-
8.56
3.89
5.63
1.06
1.89
1.76
1.5
1.63
1.8
1.77
1.04
0.61
1.11
1.22
1.55
1.79
1.24
1.46
1.05
1.27
-
7.56
2.93
2.33
6.75
1.61
3.13
2.28
2.94
pmol/ug DNA
     (BCH081209) PS(36:4) -
-
-
12.7
7.14
8.26
8.12
8.23
9.48
8.46
7.22
8.69
9.5
7.85
8.27
8.23
8.85
8.51
8.6
7.93
9.37
7.77
9.18
8.95
8.57
10.8
8.5
10.8
9.95
11.1
13.2
10.4
pmol/ug DNA
     (BCH081021) PS(36:5) -
-
-
11.2
9.15
4.63
5.05
17.5
19.3
12.6
16.2
20.5
15.1
15
13
9.89
9.69
17.4
15.5
12.9
14.9
12.3
6.67
12.3
20.1
15.1
17.3
14.5
13.1
16.5
6.7
14.1
pmol/ug DNA
     (BCH081028) PS(36:5) -
-
-
9.96
10.5
7.69
5.73
6.12
5.46
4.95
5.39
4.95
5.83
4.75
4.38
4.91
3.61
4.93
5.81
5.81
5.15
3.59
4.64
4.38
11.3
5.12
5.33
8.86
5.39
5.73
4.36
4.52
pmol/ug DNA
     (BCH081209) PS(36:5) -
-
-
4
2.17
2.76
2.72
2.7
2.89
2.68
2.29
2.86
3
2.43
2.73
3.13
3.08
3
2.89
2.38
2.9
2.57
2.87
3.15
2.67
3.29
2.5
3.65
3.22
3.87
4.29
3.27
pmol/ug DNA
     (BCH081021) PS(38:0) -
-
-
3.66
40.4
1.48
2.44
6.74
32.5
32.4
39.4
33.7
12.7
5.53
20.3
30.9
10
0.24
-
8.59
12.7
1.72
2.11
-
43.6
16.2
17.6
-
104
106
38.4
21.3
pmol/ug DNA
     (BCH081028) PS(38:0) -
-
-
6.02
1.88
1.56
2.08
1.85
1.42
1.88
7
4.58
7.13
5.34
0.91
1.2
3.94
1.25
1.19
6.86
1.54
1.09
0.9
2.21
3.54
2.24
1.28
2.1
1.73
4.52
1.08
1.15
pmol/ug DNA
     (BCH081209) PS(38:0) -
-
-
3.78
0.8
1.19
1.04
2.53
1.11
2.88
2.42
2.65
2.69
0.86
1.24
2.74
2.48
2.47
2.78
0.97
2.7
0.78
2.54
2.67
2.26
0.87
2.18
3.04
0.89
3.38
1.23
0.84
pmol/ug DNA
     (BCH081021) PS(38:1) -
-
-
1.51
2.09
2.69
2.4
9.49
-
2.52
2.62
3.02
4.45
1.7
3.02
2.8
1.99
1.61
1.25
2.23
3.15
3.24
-
-
3.6
1.67
3.59
2.85
4.22
3.68
1.69
4.06
pmol/ug DNA
     (BCH081028) PS(38:1) -
-
-
1.28
1.98
0.31
0.88
0.95
0.96
1.43
1.55
1.33
1.5
1.34
1.48
1.46
1.33
1.87
1.57
1.45
2.05
0.91
1.51
1.25
0.98
1.43
0.66
2.06
0.72
-
0.97
1.14
pmol/ug DNA
     (BCH081209) PS(38:1) -
-
-
4.81
3.19
3.48
3.39
3.26
4.42
3.85
3.12
3.31
4.18
3.23
3.54
3.81
3.69
3.34
4.06
3.52
3.5
3.23
3.39
3.15
2.92
3.99
2.99
4.64
4.6
5.27
3.96
2.67
pmol/ug DNA
     (BCH081021) PS(38:2) -
-
-
8.63
7.57
9.61
6.43
9.45
5.73
9.89
9.22
9.4
7.31
8.02
8.62
8.99
6.35
6.36
9.1
8.36
9.89
8.46
4.61
4.79
9.18
8.28
9.44
9.18
10.3
14.4
6.23
12.4
pmol/ug DNA
     (BCH081028) PS(38:2) -
-
-
0.5
5.97
4.11
0.64
0.47
2.93
3.66
4.5
4.03
3.76
3.98
3.71
3.53
3.66
4.04
4.63
4.58
4.09
2.9
4.39
3.58
3.28
3.53
3.1
-
0.69
9.33
0.91
0.89
pmol/ug DNA
     (BCH081209) PS(38:2) -
-
-
16.7
11.1
13.7
13.4
12.8
15.5
13.4
11.3
14.1
16
12.6
14.6
13.9
13.8
13.7
14.5
12.1
13.8
12.3
14.5
14.7
12.7
15.9
14.5
19.2
17
20.8
19.2
18.4
pmol/ug DNA
     (BCH081021) PS(38:3) -
-
-
29.8
22.1
33.2
27
27.4
16.4
25.1
29.8
27.3
20.5
24.9
27.2
29.3
20.9
22.8
26.9
24.1
31.3
25
19
14.1
26.5
22.5
31.6
33.1
44.1
46.2
17.9
38
pmol/ug DNA
     (BCH081028) PS(38:3) -
-
-
-
14.2
8.31
-
-
5.31
7.48
8.63
8.74
7.23
9.07
7.67
6.31
5.95
6.3
8.57
7.7
8.02
5.35
9.04
7.26
6.3
5.64
7.25
4.34
0.9
0.55
1.1
1.95
pmol/ug DNA
     (BCH081209) PS(38:3) -
-
-
52.3
34
42
40.6
42.2
45.6
42.8
34.9
44.1
46.4
39.1
42.7
44.4
42.7
43.3
43.3
40
46.5
38.5
43.3
44.1
37.9
47.6
42.6
57.9
52.8
62.3
54.9
49
pmol/ug DNA
     (BCH081021) PS(38:4) -
-
-
63.1
63.6
83.2
58.4
48.2
53.6
65.5
68.1
70.1
53.2
64.9
66.6
72.3
54.1
57.7
64
58.9
75.6
68.4
44.9
39.5
75.7
64.4
76.2
81.7
107
118
43.2
97.9
pmol/ug DNA
     (BCH081028) PS(38:4) -
-
-
78
103
69.2
72.2
77.6
63.8
80
84.5
59.4
90.2
82
68.2
74.4
68.9
93.9
84.6
99.8
89.5
63.2
83.6
86.9
78
86.2
59.7
54.5
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(38:4) -
-
-
106
58.9
70.5
70
71.8
81
76.2
64.8
74.7
78
65.9
70.3
74
71.8
71.6
71.8
70.3
77.7
64.5
74.3
73.4
61.3
79.7
64.6
93.4
83.2
97.4
86.6
83.8
pmol/ug DNA
     (BCH081021) PS(38:5) -
-
-
19.1
16.5
28.7
17.8
19.5
15.3
22.3
23.1
24.1
14.5
19.6
18.9
21.3
19.6
20.1
18.9
18.9
20.8
20.7
18.4
13.7
20.2
20.5
25.8
26
35.9
28.7
17.6
29.3
pmol/ug DNA
     (BCH081028) PS(38:5) -
-
-
28.6
30.6
21.7
22.8
-
18.4
24.8
25.4
16
28
23.3
19
21.5
19.3
27.2
24.4
28.1
26.8
17.5
24.4
27.6
21.6
23.9
19.7
15.9
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(38:5) -
-
-
38.6
19.3
24.2
23.2
24.5
26.7
25.9
21.7
25.3
26.7
22.5
24.3
24.6
25.3
24.5
26.3
24
25.7
20.8
27.3
25.7
21.8
28.2
23.5
33.4
29.1
32.4
35.7
23.2
pmol/ug DNA
     (BCH081021) PS(38:6) -
-
-
6.17
6.66
7.41
5.25
11.9
6.92
7.07
9.53
-
4.62
6.59
6.92
7.25
6.43
-
8.1
7.1
9.03
6.14
5.46
4.52
9.19
7.56
9.33
8.66
12.7
12.1
5.17
10.1
pmol/ug DNA
     (BCH081028) PS(38:6) -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(38:6) -
-
-
10.2
6.35
7.71
7.39
7.84
8.85
7.95
6.71
8.12
8.81
8.26
8.27
8.77
9.46
7.98
8.87
7.32
9.44
7.3
9.5
9.31
8.44
9.66
8.58
12.7
9.53
10.3
12.9
11.1
pmol/ug DNA
     (BCH081021) PS(40:1) -
-
-
0.64
-
-
2.49
-
0.43
0.31
-
1.01
0.22
-
-
-
2.35
5.27
4.43
2.1
-
4.03
5.7
4.48
-
-
-
-
-
-
-
-
pmol/ug DNA
     (BCH081028) PS(40:1) -
-
-
2.39
25
15
16.2
0.74
3
8.6
0.99
3.94
0.77
8.26
6.64
2.68
0.56
0.08
1.53
-
2.2
-
0.53
-
1.47
1.49
1.69
2.01
1.45
1.01
1.12
0.92
pmol/ug DNA
     (BCH081209) PS(40:1) -
-
-
1.03
0.62
0.97
0.98
0.83
0.9
0.8
0.68
0.93
0.84
0.7
1
0.86
0.72
0.75
0.61
0.69
0.71
0.94
0.77
0.88
0.87
1.01
0.88
0.83
1.14
1.03
1.07
1.09
pmol/ug DNA
     (BCH081021) PS(40:2) -
-
-
0.67
12.6
27.3
5.02
3.9
27.9
26.4
29.8
26.1
13
3.55
16.2
25.8
9.06
0.13
0.08
6.81
11.5
-
-
-
36.9
12.8
15.5
-
83.7
85
23.3
22.6
pmol/ug DNA
     (BCH081028) PS(40:2) -
-
-
1.1
-
-
5.69
0.91
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
0.2
-
-
-
0.29
2.21
2.12
1.24
-
pmol/ug DNA
     (BCH081209) PS(40:2) -
-
-
-
0.01
-
-
-
0.27
-
-
-
-
-
-
-
0.37
-
0.19
-
-
-
-
-
-
-
-
-
-
-
-
-
pmol/ug DNA
     (BCH081028) PS(40:3) -
-
-
1.99
4.61
2.3
2.75
4.3
1.93
1.28
3.16
2.77
1.84
2.49
2.58
2.76
1.24
3.82
4.52
2.6
2.79
1.51
2.5
2.4
1.55
2.58
2.3
1.94
-
-
-
12.8
pmol/ug DNA
     (BCH081209) PS(40:3) -
-
-
3.97
4.44
5.58
5.79
3.08
4.97
5.37
2.83
5
5.41
4.59
4.32
4.01
4.33
5.49
7.08
4.56
3.33
4.46
3.55
6.96
6.55
6.64
5.01
7.59
8.3
9.96
9.29
4.45
pmol/ug DNA
     (BCH081028) PS(40:4) -
-
-
55.9
91.6
55.8
58.2
60.1
47.7
62.5
69.5
56.4
65.1
66.7
55.9
55.9
52.9
64.5
62.9
76.3
74.1
46.9
71.6
65.7
60
67.5
55
45
-
-
-
-
pmol/ug DNA
     (BCH081209) PS(40:4) -
-
-
89.9
63.5
76.6
74
75.4
89.5
74.3
65.4
82.3
94.1
73.6
81.2
82.5
84
83.9
83.6
66.7
85.2
74.7
81.2
84.3
82.9
98.7
92.7
117
111
123
110
75.6
pmol/ug DNA
     (BCH081028) PS(40:5) -
-
-
41.3
58.1
37
42.6
45.4
31.2
39
42.5
34.2
48.6
44.7
37.2
36.9
37
45.4
47.3
49.6
51.5
33.7
47.8
47.5
43.7
44
36.7
30.7
4.71
4.57
3.07
3.35
pmol/ug DNA
     (BCH081209) PS(40:5) -
-
-
66.4
44.3
52.6
49.7
52.9
58.9
54.1
45.8
55.1
57.6
50.7
51.1
58.8
54.5
51.5
56.3
51.5
63.9
48.2
61.3
55.5
53.8
62.2
59.8
78.8
72.1
81.3
75.8
55.9
pmol/ug DNA
     (BCH081028) PS(40:6) -
-
-
4.45
26.5
17.2
2.16
2.08
13.9
18.4
18.8
15.5
20
19.3
16.5
16.2
14.8
19
20.8
21.8
20.3
13.2
20.2
19.9
16.2
18.7
16.8
12
1.07
1.58
1.24
1.32
pmol/ug DNA
     (BCH081209) PS(40:6) -
-
-
64.6
37.2
47.4
45.8
45.8
52.5
48.5
40.9
48.3
51.6
44.4
47.3
48.3
48.9
48.8
49.4
45.6
50.8
42.2
53.1
50
42.6
51.7
53
66.9
64.2
69.6
74.4
62.4
pmol/ug DNA
     (BCH081028) PS(40:7) -
-
-
6.29
6.04
6.76
5.62
5.17
5.15
4.83
5.82
2.62
4.29
3.83
3.85
4.47
2.57
3.85
3.72
4.33
3.57
1.99
3.08
2.88
4.53
2.91
2.12
2.76
3.71
2.93
1.42
2
pmol/ug DNA
     (BCH081209) PS(40:7) -
-
-
12.9
5.77
7
6.68
7.33
7.94
7.51
6.66
7.92
8.02
6.79
7.14
7.43
7.83
7.44
7.98
7.36
9.04
7.03
8.5
7.37
7.21
8.98
7.22
10.3
10.7
11.5
10.9
6.74
pmol/ug DNA