Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin

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PA| PC| PE| PG| PI| PS| DNA| TNF

Details/Graph
LM_ID Name Kdo+Comp
Kdo
Comp
Ctrl
 0 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 0.5 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 1 hr		 Kdo+Comp
Kdo
Comp
Ctrl
 2 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 4 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 8 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 12 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 24 hrs		 Units
. . . .
     (BCH081021) PG(32:0) -
-
-
-		 1.79
1.58
1.13
-		 2.5
2.69
2.42
2.35		 1.4
1.55
2.21
2.38		 2.67
2.35
2.59
2.75		 1.59
1.82
2.34
2.05		 1.81
2.01
2.7
2.86		 2.61
3.84
1.86
3.88		 pmol/ug DNA
     (BCH081028) PG(32:0) -
-
-
0.74		 1.05
0.68
0.66
0.72		 0.63
0.86
0.82
0.88		 0.79
0.71
0.73
0.82		 0.75
0.88
0.9
0.9		 0.96
0.83
1.07
0.97		 0.79
1.19
1.13
1.29		 1.82
1.81
1.15
1.33		 pmol/ug DNA
     (BCH081209) PG(32:0) -
-
-
1.97		 1.22
1.53
1.54
1.42		 1.37
1.38
1.1
1.49		 1.39
1.19
1.21
1.4		 1.35
1.54
1.47
1.38		 1.46
1.23
1.5
1.68		 1.68
2.4
1.78
2.64		 3.51
4.8
2.5
2.67		 pmol/ug DNA
     (BCH081021) PG(32:1) -
-
-
1.04		 1.61
2.29
-
5.61		 1.71
2.13
2.33
1.93		 1.2
1.41
1.38
1.82		 2.41
2.23
2.19
2.02		 1.64
2.2
-
3.37		 1.27
1.21
1.69
2.36		 1.59
1.46
1.15
1.79		 pmol/ug DNA
     (BCH081028) PG(32:1) -
-
-
0.41		 0.98
0.5
0.61
0.76		 0.47
0.55
0.7
0.71		 0.72
0.54
0.56
0.63		 0.79
0.97
0.95
0.67		 1.11
0.8
0.67
1.03		 1.56
1.71
1.12
0.6		 1.03
1.1
0.73
0.93		 pmol/ug DNA
     (BCH081209) PG(32:1) -
-
-
0.82		 0.48
0.36
0.47
0.68		 0.49
0.32
0.46
0.52		 0.46
0.42
0.49
0.6		 0.69
0.69
0.61
0.54		 0.71
0.6
0.69
0.85		 0.9
1.07
0.79
1.48		 1.41
1.62
2.68
1.32		 pmol/ug DNA
     (BCH081021) PG(34:1) -
-
-
7.03		 4.35
8.95
5.08
7.82		 4.19
4.22
3.91
4.28		 3.38
5.27
5.26
4.98		 6.64
4.84
5.31
4.98		 4.02
6.34
6.84
4.54		 4.36
3.04
6
9.27		 5.26
7.01
7.73
9.21		 pmol/ug DNA
     (BCH081028) PG(34:1) -
-
-
3.49		 4.75
2.87
2.56
3.08		 2.58
2.99
3.42
3.98		 3.33
3.35
3.28
3.16		 3.42
4.11
4.73
3.89		 4.05
3.04
5.11
4.79		 3.77
4.87
4.81
4.52		 6.42
6.58
5.12
4.74		 pmol/ug DNA
     (BCH081209) PG(34:1) -
-
-
9.78		 5.9
7.32
8.53
6.81		 7.31
6.75
6.13
7.38		 7.51
6.18
7.02
7.32		 7.08
7.02
7.96
6.12		 6.74
6.02
7.8
7.88		 7.93
9.44
8.99
11.1		 12.3
18.1
4.59
2.59		 pmol/ug DNA
     (BCH081021) PG(34:2) -
-
-
4.04		 5.18
8.95
6.09
4.38		 6.76
7.22
7.9
6.96		 4.65
4.96
5.68
6.11		 7.38
6.92
8.1
7.98		 5.83
6.42
6.44
5.84		 5.32
4.75
7.5
9.57		 7.31
7.26
6.66
9.71		 pmol/ug DNA
     (BCH081028) PG(34:2) -
-
-
0.26		 4.02
2.4
2.96
3.48		 2.38
2.78
3.44
2.67		 3.38
2.82
2.64
3.04		 2.93
3.76
3.61
3.84		 3.28
2.8
2.88
2.77		 4.08
3.39
3.14
3.81		 4.63
0.84
1.03
4.23		 pmol/ug DNA
     (BCH081209) PG(34:2) -
-
-
3.35		 2.15
2.72
1.07
0.93		 2.14
2.25
1.9
1.37		 2.31
1.54
1.72
2.14		 1.16
1.85
2.42
1.96		 1.46
1.24
1.96
1.7		 1.22
2.23
2.35
2.36		 3.99
2.56
2.89
2.83		 pmol/ug DNA
     (BCH081021) PG(34:3) -
-
-
8.84		 12.8
21.7
15
10.2		 16.5
20.4
20.5
15.5		 12.1
13
14.8
17.9		 19.3
17.9
21
19.8		 14.8
16.9
15.7
14.4		 13.4
10.9
18
22.6		 18.3
17.2
15
21.8		 pmol/ug DNA
     (BCH081028) PG(34:3) -
-
-
6.35		 7.64
4.96
5.49
6.44		 4.72
5.37
6.24
5.83		 6.57
5.46
5.24
5.72		 4.78
7.2
7.7
6.58		 7.5
5.7
5.66
6.18		 6.32
8.48
6.33
6.57		 11.7
9.76
6.25
8.73		 pmol/ug DNA
     (BCH081209) PG(34:3) -
-
-
1.01		 0.66
0.61
0.55
0.54		 0.62
0.58
0.51
0.63		 0.55
0.48
0.6
0.55		 0.6
0.57
0.67
0.49		 0.72
0.54
0.51
0.57		 0.35
0.52
0.6
0.85		 0.48
0.69
1.22
0.92		 pmol/ug DNA
     (BCH081021) PG(36:1) -
-
-
7.32		 1.85
5.34
4.63
5.88		 2.47
3.8
4.03
2.62		 1.28
2.78
4.39
4.33		 4.43
2.69
3.87
2.53		 1.29
3.32
3.23
1.66		 0.79
1.76
6.04
6.98		 5.71
3.11
2.86
5.25		 pmol/ug DNA
     (BCH081028) PG(36:1) -
-
-
0.96		 3.39
3.17
3.25
4.06		 2.04
3.37
2.71
2.31		 2.64
3.23
3.1
2.99		 1.8
2.41
3.41
3.95		 2.14
1.33
2.13
2.2		 0.69
1.9
1.91
0.79		 3.13
1.75
3.2
1.35		 pmol/ug DNA
     (BCH081209) PG(36:1) -
-
-
4.08		 2.6
3.35
1.32
5.67		 3.45
1.76
2.29
3.13		 3.2
2.69
3.14
3.79		 3.47
2.92
3.22
2.35		 3.11
2.83
2.72
3.11		 2.7
2.73
3.63
4.83		 4.22
11.2
9.42
5.59		 pmol/ug DNA
     (BCH081021) PG(36:2) -
-
-
8.21		 27.7
30.6
21.5
17.3		 23.2
27.3
26.5
21.7		 19.2
20.4
18.1
32.4		 33.7
25.2
28
39.1		 25.2
27.9
26.2
25.6		 27.5
18.9
35.8
40.1		 23.2
28.1
51.7
61.3		 pmol/ug DNA
     (BCH081028) PG(36:2) -
-
-
9.72		 14.7
8.66
6.82
8.18		 9.92
8.13
13.7
9.24		 13
7.36
7.06
10.4		 8.4
14.1
9.42
8.91		 11.6
9.14
9.39
9.77		 8.02
13.9
8.59
8.58		 13.3
13.2
10.9
13.9		 pmol/ug DNA
     (BCH081209) PG(36:2) -
-
-
44.7		 21.8
23.8
29.6
21.8		 23.2
29.7
17.7
23.2		 22.5
18.7
22.7
24.5		 25
21.8
23
18.6		 23.3
19.5
22
24.2		 18.1
19.2
26.1
35.2		 29.7
57.1
63.4
36.7		 pmol/ug DNA
     (BCH081021) PG(36:3) -
-
-
5.44		 31.8
43.3
27.8
3.62		 38.8
41.1
40.6
34.8		 19.6
28.9
29.5
34.3		 45.7
37.2
37.7
37.6		 31.2
36.4
36.1
23.8		 22.9
22.2
41
44.6		 21.8
27.2
28.5
44.9		 pmol/ug DNA
     (BCH081028) PG(36:3) -
-
-
8.65		 9.15
7.41
5.63
6.15		 7.15
7.65
9.88
7.67		 8.67
5.98
6.21
6.27		 7.44
9.55
8.17
8.03		 7.72
5.8
8.37
8.59		 4.37
7.73
6.88
5.95		 6.19
6.15
5.28
7.03		 pmol/ug DNA
     (BCH081209) PG(36:3) -
-
-
54.7		 25.5
29.5
22.7
21.5		 28.6
25.3
20.7
22.1		 26.1
21.4
25.9
27.8		 21.3
23.7
25.4
20.1		 19.1
17
22.1
19		 14.2
16.5
23
24		 27.4
25.9
30.1
17.6		 pmol/ug DNA
     (BCH081021) PG(36:4) -
-
-
6.02		 10.1
9.34
5.51
6.39		 10
10.9
10.9
8.87		 1.98
7.19
5.61
7.47		 19.7
6.81
8.03
7.05		 3.43
8.79
8.29
3.67		 1.06
2.3
5.98
5.41		 -
-
-
0.48		 pmol/ug DNA
     (BCH081028) PG(36:4) -
-
-
-		 7.58
3.68
3.27
3.94		 1.95
2.79
3.62
3.11		 1.98
1.93
2.44
2.72		 4.07
4.74
2.26
3.96		 -
0.08
1.15
4.32		 8.72
8.5
0.57
6.43		 2.73
5.28
4.56
6.06		 pmol/ug DNA
     (BCH081209) PG(36:4) -
-
-
22.5		 10.5
12.2
9.23
8.73		 11.5
11
9.24
10.5		 12
8.76
10.9
11.6		 7.7
10.1
10.3
8.64		 9.59
5.89
8.01
9.58		 4.77
5.45
5.33
7.98		 11.3
12.8
7.55
4.37		 pmol/ug DNA
     (BCH081021) PG(36:5) -
-
-
46.4		 45.1
76.3
59.9
61.6		 61
59.6
63.9
65.4		 49.6
47.9
56.2
59.5		 -
64
73.2
71.6		 75.1
56.4
52.9
48.5		 65.9
53.8
83
105		 92.5
88.5
75
106		 pmol/ug DNA
     (BCH081028) PG(36:5) -
-
-
35.7		 41.1
26.9
30.4
35.3		 26.1
30.6
36.5
29.8		 33.7
27.8
28
30.3		 22.4
29.3
30.4
34.1		 28.4
19.6
22.9
26		 32.7
32.1
20.8
23.5		 42.6
37.5
17
27.3		 pmol/ug DNA
     (BCH081209) PG(36:5) -
-
-
2.02		 1.06
1.04
0.85
0.79		 1.04
0.94
0.78
0.99		 1.08
0.85
1.19
1.24		 0.95
1.02
0.84
0.81		 1.27
0.85
0.97
0.97		 0.81
1.01
1
1.5		 0.76
0.72
1.45
1.44		 pmol/ug DNA
     (BCH081021) PG(38:1) -
-
-
0.79		 0.08
0.89
0.49
0.93		 0.75
0.48
0.55
0.85		 0.42
0.36
0.55
0.66		 0.72
0.7
0.6
0.2		 0.33
0.6
0.52
0.5		 0.43
0.42
0.79
1.01		 0.81
0.66
0.5
0.75		 pmol/ug DNA
     (BCH081028) PG(38:1) -
-
-
0.6		 1.21
0.67
0.95
0.74		 0.53
0.58
0.61
0.48		 0.53
0.71
0.6
0.6		 0.63
0.69
0.55
0.7		 0.74
0.5
0.64
0.22		 0.34
0.52
0.33
1.66		 0.81
0.68
0.5
0.56		 pmol/ug DNA
     (BCH081209) PG(38:1) -
-
-
0.23		 0.21
0.18
0.18
0.31		 0.15
0.16
0.11
0.22		 0.23
0.17
0.16
0.31		 0.3
0.31
0.2
0.1		 0.28
0.25
0.18
0.35		 0.36
0.11
0.18
0.25		 0.57
0.6
0.19
0.62		 pmol/ug DNA
     (BCH081021) PG(38:2) -
-
-
1.73		 4.83
1.82
1.56
0.5		 1.24
2.05
1.87
-		 0.87
0.86
1.26
1.65		 2.2
1.33
1.68
3.73		 0.71
1.44
0.84
0.45		 0.75
0.63
1.61
1.74		 1.52
1.55
1.56
2.08		 pmol/ug DNA
     (BCH081028) PG(38:2) -
-
-
0.37		 0.39
0.38
0.32
0.93		 0.3
0.33
1.17
3.28		 1.4
0.25
0.91
1.12		 0.69
1.28
1.49
1.18		 0.41
0.9
0.35
2.74		 0.44
2.1
1.29
0.46		 0.57
0.76
0.43
0.55		 pmol/ug DNA
     (BCH081209) PG(38:2) -
-
-
2.07		 1.5
1.96
1.66
0.96		 1.87
1.7
1.28
1.83		 1.81
1.44
1.73
2.03		 2.65
1.79
2.15
1.41		 1.66
1.62
1.67
1.75		 1.83
2.44
2.02
2.61		 2.87
3.38
4.83
3.33		 pmol/ug DNA
     (BCH081021) PG(38:3) -
-
-
1.68		 2.55
3.13
2.62
1.86		 2.97
3.11
2.87
2.7		 1.39
1.81
2.39
2.89		 3.5
2.72
3.28
3.45		 2.5
2.59
4.1
1.94		 1.87
2.3
3.97
3.46		 2.23
3.35
2.62
4.77		 pmol/ug DNA
     (BCH081028) PG(38:3) -
-
-
1.36		 1.95
1.53
1.22
1.28		 1.1
1.33
1.62
1.41		 1.3
1.1
1.11
1.26		 1.14
1.35
1.41
1.38		 1.12
0.88
1.11
1.23		 0.85
1.32
1.1
1.15		 1.4
1.45
1.21
1.43		 pmol/ug DNA
     (BCH081209) PG(38:3) -
-
-
8.71		 5.04
5.68
4.51
5.91		 5.35
5.18
4.42
5.52		 4.97
4.2
5.39
5.42		 4.88
4.63
5.07
4		 4.9
4.43
4.73
4.37		 5.15
4.03
5.48
6		 6.08
5.49
13.1
8.05		 pmol/ug DNA
     (BCH081021) PG(38:4) -
-
-
7.96		 7.27
7.13
6.44
5.66		 6.56
7.94
6.52
6.94		 4.19
5.44
6
6.92		 8.3
8.73
7.01
7.33		 6.59
6.04
6.45
4.05		 5.2
4.2
7.86
10.8		 5.73
6.48
6.91
8.13		 pmol/ug DNA
     (BCH081028) PG(38:4) -
-
-
5.25		 5.21
3.5
2.92
3.37		 3.35
3.94
4.62
4.23		 4.19
3.11
3.17
3.3		 3.07
4.71
4.81
3.95		 3.18
2.75
3.6
4.14		 2.08
3.68
3.54
2.24		 2.57
3.41
3.39
3.83		 pmol/ug DNA
     (BCH081209) PG(38:4) -
-
-
21.1		 10.1
11.1
8.79
14.4		 11.7
9.22
8.75
11.1		 10.7
8.76
10.5
11.8		 15.4
9.98
10.7
8.39		 10.6
8.91
9.4
14.3		 12.3
8.63
10.6
19.6		 11.3
23.5
23.5
15.7		 pmol/ug DNA
     (BCH081021) PG(38:5) -
-
-
13.1		 13.2
24
19.1
21.2		 20.7
18.7
22.7
21.5		 17.3
14
16.1
16.9		 18.4
18.6
22.8
19.1		 24
16.6
16.6
14.6		 19.3
17.7
19.9
31.2		 25.1
30.8
18.6
26.2		 pmol/ug DNA
     (BCH081028) PG(38:5) -
-
-
6.56		 13
12.9
9.44
9.28		 5.48
6.47
7.78
11.1		 7.27
5.73
5
6.2		 5.29
2.86
2.59
7.79		 4.95
1.24
14.7
5.94		 8.32
6.52
1.64
12		 11.8
9.26
3.71
5.07		 pmol/ug DNA
     (BCH081209) PG(38:5) -
-
-
27.6		 12.7
16.2
15
12.4		 13.6
15.4
10.5
13.7		 14.8
11.2
13.9
13.7		 13.8
13.1
12.8
11		 14.6
12.1
12.3
12.4		 10.1
10.7
13.1
17.1		 12.5
20.2
22.4
15.2		 pmol/ug DNA
     (BCH081021) PG(38:6) -
-
-
30.4		 5.99
10.8
8.24
25.4		 14.2
6.7
7.22
8.73		 6.22
6.33
5.37
6.45		 7.89
7.63
6.69
8.57		 10.1
14
5.6
5.88		 8.16
7.51
53.7
13.4		 12.9
13.9
9.69
11.5		 pmol/ug DNA
     (BCH081028) PG(38:6) -
-
-
3.83		 54.2
9.21
42.1
44.7		 26.9
28.9
44.2
7.77		 30.2
31.4
35.8
35.1		 2.92
41.2
37.5
30.7		 38.7
26.2
6.44
3.65		 29.5
37.7
27.1
22.6		 11.7
8.71
4.08
7.16		 pmol/ug DNA
     (BCH081209) PG(38:6) -
-
-
13.7		 7.83
8.16
7.16
6.52		 8.4
7.97
6.46
7		 8.36
6.13
7.56
7.98		 6.69
6.89
7.49
5.86		 6.27
5.59
7.05
7.23		 4.4
4.84
6.71
8.55		 7.33
9.04
10.3
6.53		 pmol/ug DNA
     (BCH081021) PG(40:3) -
-
-
2.66		 0.17
1.22
2.31
0.01		 -
0.71
-
-		 2.7
3.41
-
-		 1.96
0.41
-
-		 1.33
0.67
1.02
0.55		 0.54
2.44
1.57
1.84		 1.35
0.75
3.49
0.8		 pmol/ug DNA
     (BCH081028) PG(40:3) -
-
-
0.33		 2.68
2.68
2.29
1.15		 1.4
0.83
2.29
4.1		 0.22
0.08
0.29
0.33		 0.73
0.67
0.17
0.09		 0.55
0.46
-
0.28		 3.46
-
0.59
3.78		 1.16
0.71
0.86
1.22		 pmol/ug DNA
     (BCH081209) PG(40:3) -
-
-
0.82		 0.8
0.7
0.63
0.56		 0.95
0.9
0.55
1.12		 0.62
0.58
0.49
0.85		 1.04
0.81
0.86
0.6		 0.59
0.51
0.87
0.42		 0.33
0.81
1.1
1.38		 0.9
1.27
1.08
0.5		 pmol/ug DNA
     (BCH081021) PG(40:4) -
-
-
2.64		 0.37
0.65
0.57
2.44		 0.71
1.22
0.85
-		 0.07
-
-
-		 0.66
0.68
0.81
0.49		 0.12
-
1.44
1.02		 0.21
0.33
0.38
-		 0.87
1.04
2.77
-		 pmol/ug DNA
     (BCH081028) PG(40:4) -
-
-
0.89		 1.62
0.64
0.62
0.54		 0.48
0.9
1.21
1.02		 0.61
0.5
0.54
0.58		 0.53
1.21
0.35
0.6		 1.38
0.51
0.45
1.95		 0.41
0.65
0.7
0.69		 1.27
0.95
0.46
1.07		 pmol/ug DNA
     (BCH081209) PG(40:4) -
-
-
4.28		 3.97
4.62
3.54
2.93		 4.45
3.75
3.51
3.78		 4.88
3.3
4.02
5.59		 4.91
4.13
4.2
3.25		 4.08
2.77
3.4
3.98		 4.53
4.49
4.36
5.72		 5.92
6.61
5.57
4.74		 pmol/ug DNA
     (BCH081021) PG(40:5) -
-
-
1.99		 8.01
10.1
8.18
-		 8.09
10.3
9.22
8.12		 6.23
6.66
8.89
11.3		 11.5
10.2
9.69
11.3		 7.53
8.94
8.6
6.25		 8.55
8.54
12.6
14.7		 10.5
12.2
-
15.4		 pmol/ug DNA
     (BCH081028) PG(40:5) -
-
-
3.96		 6.31
4.31
4.07
3.83		 3.2
3.85
4.5
3.75		 3.52
3.18
3.44
3.73		 2.47
2.94
3.71
3.65		 2.58
2.08
2.92
2.83		 1.85
3.07
2.22
2.18		 2.73
3.19
2.22
2.95		 pmol/ug DNA
     (BCH081209) PG(40:5) -
-
-
23.8		 16.9
17.6
16.7
15		 19.4
16.5
13.7
18.6		 16.8
13.9
17.8
18.5		 17.2
15.6
17.7
14		 16.4
14.8
13.4
15.8		 11.6
13.5
16.8
20.2		 24.2
30.7
25.8
18.2		 pmol/ug DNA
     (BCH081021) PG(40:6) -
-
-
-		 23.3
36.7
30.1
-		 27.9
28.9
28
26.5		 18.4
23.9
29.2
39		 33.4
28.4
29.9
32.4		 25.5
29.7
23.6
17.3		 22.7
26.1
38.2
48		 36.1
36.6
25.5
37.5		 pmol/ug DNA
     (BCH081028) PG(40:6) -
-
-
9.64		 15.7
9.94
9.26
9.56		 7.58
8.9
10.6
8.26		 7.76
7.87
8.22
8.23		 4.97
6.9
8.78
8.3		 5.46
4.24
6.69
5.95		 4.77
6.96
4.55
4.25		 6.37
6.75
3.69
5.22		 pmol/ug DNA
     (BCH081209) PG(40:6) -
-
-
67.2		 41.9
47.8
41
37.1		 48.7
41.8
34.8
42.5		 43.9
35.3
43.8
47.8		 40.3
40
42.8
33.3		 36.6
32.8
34.2
35.7		 28.1
33
37.2
47.7		 55.9
65.5
52.1
36.4		 pmol/ug DNA
     (BCH081021) PG(40:7) -
-
-
44.6		 29
45
38
38.1		 34.8
37.4
35.9
31.7		 21.5
30.5
34.4
42.7		 41
34.8
38
40		 33.5
36.4
30.7
20.8		 26.6
30.7
44.2
53.4		 42.2
41.5
26.3
44.7		 pmol/ug DNA
     (BCH081028) PG(40:7) -
-
-
23.2		 29
21.3
17.6
19.6		 17.3
20.8
25.6
20.2		 20.5
16.3
17.4
17.9		 13.3
18.2
21.1
20.9		 14.9
11.6
17.4
16.2		 12.7
19.8
13.2
12.4		 20.7
20.5
8.87
13.3		 pmol/ug DNA
     (BCH081209) PG(40:7) -
-
-
142		 76.2
84
74.1
68.9		 84.2
76
62.3
77.9		 78.4
61.3
76.6
80.5		 73.1
69.7
76.2
62		 72.7
59.4
63.1
65.8		 50.1
58.7
64.2
85.4		 90.6
104
94.3
63.7		 pmol/ug DNA