Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A and compactin

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PA| PC| PE| PG| PI| PS| DNA| TNF

Details/Graph
LM_ID Name Kdo+Comp
Kdo
Comp
Ctrl
 0 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 0.5 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 1 hr		 Kdo+Comp
Kdo
Comp
Ctrl
 2 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 4 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 8 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 12 hrs		 Kdo+Comp
Kdo
Comp
Ctrl
 24 hrs		 Units
. . . .
     (BCH081021) PA(32:0) -
-
-
4.29		 5.41
6.14
3.15
3.79		 9.18
8.25
7.07
6.64		 4.97
4.96
5.52
5.51		 9.98
8.51
8.44
8.24		 9.98
8.01
7.16
7.89		 10.5
7.7
8.03
8.63		 11.9
18.6
5.21
11.1		 pmol/ug DNA
     (BCH081028) PA(32:0) -
-
-
2.51		 7.27
4.6
3.08
4.3		 3.57
3.49
3.9
5.93		 3.75
4.5
3.77
2.82		 4.86
5.3
5.36
4.82		 11.7
6.14
6.28
6.07		 6.54
9.32
7.97
9.46		 7.87
10
7.18
8.54		 pmol/ug DNA
     (BCH081209) PA(32:0) -
-
-
3.76		 4.25
4.55
4.16
3.67		 4.88
4.48
3.5
4.34		 4.94
3.89
4.18
4.28		 5.5
5.81
4.59
3.52		 5.57
5.09
4.61
5.36		 6.62
8.11
6.57
7.78		 6.81
8.65
11.5
13.5		 pmol/ug DNA
     (BCH081021) PA(32:1) -
-
-
-		 -
-
-
-		 -
-
-
-		 2.07
1.89
1.7
-		 4.85
3.75
3.34
3.25		 3.68
3.58
-
-		 4.56
3.18
3.32
3.32		 5.31
7.89
2.27
4.93		 pmol/ug DNA
     (BCH081028) PA(32:1) -
-
-
1.04		 2.56
1.46
0.97
1.55		 1.16
1.74
1.34
1.69		 1.6
1.24
1.28
1.36		 2
-
-
-		 -
-
-
-		 3.02
-
-
3.66		 3.45
4.44
4.05
4.15		 pmol/ug DNA
     (BCH081209) PA(32:1) -
-
-
1.34		 1.43
1.72
1.61
1.66		 1.91
2.03
1.48
1.78		 2.04
1.6
1.59
1.67		 2.31
2.16
2.01
1.55		 2.48
2.29
1.98
2.27		 2.8
3.07
2.62
3.23		 2.36
3.58
5.69
5.8		 pmol/ug DNA
     (BCH081021) PA(34:0) -
-
-
2.84		 3.43
3.88
2.41
5.5		 3.57
3.59
-
3.05		 2.63
2.95
2.52
2.59		 3.87
3.6
4.48
4.08		 4.18
3.39
-
3.82		 3.89
3.63
3.32
3.21		 4.45
6.27
2.07
3.53		 pmol/ug DNA
     (BCH081028) PA(34:0) -
-
-
1.86		 4.24
2.65
2.16
2.99		 2
2.54
2.85
3.37		 3.12
3
2.42
2.09		 2.64
3.37
3.42
3.67		 6.95
3.52
3.27
-		 3.92
5.65
3.61
5.43		 2.57
3.41
2.25
2.89		 pmol/ug DNA
     (BCH081209) PA(34:0) -
-
-
3		 2.11
2.36
2.03
2.03		 2.23
2.41
1.91
2.39		 2.58
2.17
2.35
2.27		 2.69
2.53
2.16
1.94		 2.7
2.38
2.28
2.44		 2.7
3.38
2.53
3.42		 2.6
3.48
3.83
3.7		 pmol/ug DNA
     (BCH081021) PA(34:1) -
-
-
4.88		 6.02
7.36
4.01
4.26		 10
9.41
8.19
7.94		 6.36
6.07
6.89
6.37		 12.5
9.62
9.78
9.39		 11.8
9.87
8.78
8.46		 12.5
8.82
9.6
9.75		 14.9
18.7
5.4
12.7		 pmol/ug DNA
     (BCH081028) PA(34:1) -
-
-
4.93		 9.2
5.77
4.32
6.08		 5.02
6.17
5.56
6.82		 6.33
5.81
5.12
4.8		 6.77
7.05
7.37
7.02		 13.7
7.35
7.59
7.86		 8.5
10.5
10.8
10		 9.79
11.7
9.2
8.68		 pmol/ug DNA
     (BCH081209) PA(34:1) -
-
-
5.94		 4.95
5.56
5.58
5.1		 7.02
6.15
5
5.82		 6.73
5.42
5.38
6		 6.81
7
5.95
5.18		 7.55
6.39
6.26
6.5		 7.15
8.62
7.06
9.48		 7.61
8.59
12.7
11.7		 pmol/ug DNA
     (BCH081021) PA(34:2) -
-
-
1.65		 2.62
2.38
1.5
-		 2.88
3.98
2.71
3.64		 2.13
1.87
2.06
2.1		 4.74
3.79
3.52
3.3		 3.86
3.5
-
3.11		 4.11
3.22
2.81
3.1		 4.11
6.25
1.59
3.64		 pmol/ug DNA
     (BCH081028) PA(34:2) -
-
-
1.27		 2.51
1.67
0.89
1.48		 1.15
1.62
1.57
1.63		 2.01
1.58
1.3
1.47		 -
2.02
1.78
1.84		 4.17
1.97
2.09
3.07		 2.41
3.28
-
3.48		 2.36
2.91
2.34
2.64		 pmol/ug DNA
     (BCH081209) PA(34:2) -
-
-
1.93		 1.67
1.93
1.89
1.73		 2.3
2.08
1.72
2.05		 2.51
1.86
1.91
2.07		 2.57
2.46
2.05
1.68		 2.56
2.4
2.33
2.23		 2.45
2.77
2.4
3.31		 2.26
2.73
3.88
3.81		 pmol/ug DNA
     (BCH081021) PA(36:0) -
-
-
-		 1.08
0.36
-
-		 2.4
1.5
0.84
2.16		 0.83
0.54
-
0.68		 0.34
0.99
0.48
1.35		 0.83
0.06
1.55
1.72		 0.58
1.3
0.19
1.14		 -
1.16
3.34
1.23		 pmol/ug DNA
     (BCH081028) PA(36:0) -
-
-
0.12		 -
-
0.05
0.04		 -
0.1
-
-		 -
0.02
0.02
-		 1.52
0.32
-
-		 -
0.24
-
1.06		 -
1.81
0.16
1.76		 0.04
0.43
0.4
0.44		 pmol/ug DNA
     (BCH081209) PA(36:0) -
-
-
1.37		 0.92
0.92
1.04
1.02		 1.13
1.13
0.94
1.1		 1.1
0.99
0.95
1.19		 1.12
1.4
1.15
0.9		 1.51
0.81
1.07
1.35		 1.16
1.38
1.38
1.73		 1.46
1.78
2.29
1.41		 pmol/ug DNA
     (BCH081021) PA(36:1) -
-
-
9.33		 5.89
9.73
5.56
14.4		 8.46
8.19
7.61
6.51		 5.7
5.94
6.13
5.78		 9.49
7.27
8.08
7.24		 9.12
8.02
5.68
6.7		 8.87
8.01
8.85
8.6		 13.4
13.8
4.75
10.6		 pmol/ug DNA
     (BCH081028) PA(36:1) -
-
-
5.53		 11.1
8.16
5.43
7.4		 6
7.95
8.32
9.31		 8.35
7.16
6.41
6.79		 7.41
9.94
9.27
9.17		 15.8
8.36
8.95
9.38		 9.48
12.6
11.6
10.8		 7.82
9.17
7.04
7.47		 pmol/ug DNA
     (BCH081209) PA(36:1) -
-
-
8.4		 4.93
5.5
5.79
5.65		 5.84
6.2
5.4
6.09		 6.24
5.2
5.68
5.79		 6.38
5.98
5.75
5.36		 6.9
5.59
6.43
6.15		 5.52
7.22
6.35
8.77		 7.25
8.52
10.1
7.64		 pmol/ug DNA
     (BCH081021) PA(36:2) -
-
-
-		 10.8
14.6
9.17
-		 14.6
15.4
13.2
14.3		 10.6
11
11.3
10.9		 16.6
13.4
14.3
14		 17.6
14.6
13.1
11.3		 17.3
13.8
15.6
15.6		 22.1
21.6
9.06
18.5		 pmol/ug DNA
     (BCH081028) PA(36:2) -
-
-
10		 16.5
12.3
8.04
11.8		 9.85
12.4
11.8
12.7		 12.3
11.8
9.23
9.28		 11.4
13.7
13.9
13.6		 24.2
12.3
14.2
14.8		 14.3
18.8
17.2
17.3		 14.1
14.6
9.67
10.9		 pmol/ug DNA
     (BCH081209) PA(36:2) -
-
-
14.7		 8.89
10.3
10.6
10.3		 11.6
11.3
10.3
11.2		 12.1
9.64
10.6
11.2		 11.7
11.1
11.6
10.4		 12.9
10.1
11.8
11.6		 10.2
12.9
11.5
15.3		 12.3
13.7
16.8
10.6		 pmol/ug DNA
     (BCH081021) PA(36:3) -
-
-
0.13		 0.88
0.45
0.35
11.4		 1.8
1.1
1.03
1.37		 0.98
0.55
0.85
1.01		 1.18
1.28
1.09
0.72		 1.43
0.86
1.83
1.58		 2
1.28
1.48
1.21		 2.17
2.51
0.86
1.47		 pmol/ug DNA
     (BCH081028) PA(36:3) -
-
-
1.67		 2.92
1.63
1.42
1.63		 1.9
2.03
2.07
1.34		 2.03
1.94
1.71
1.43		 -
1.99
2.09
1.95		 4.65
2.64
-
-		 3.27
3.55
-
-		 2.14
2.78
2.17
1.87		 pmol/ug DNA
     (BCH081209) PA(36:3) -
-
-
1.6		 1.56
1.52
1.47
1.33		 1.93
1.8
1.41
1.52		 2.04
1.61
1.53
1.69		 2.1
1.88
1.63
1.47		 2.25
1.91
1.89
1.89		 1.89
2.17
1.81
2.34		 2.09
2.49
2.56
1.81		 pmol/ug DNA
     (BCH081021) PA(36:4) -
-
-
3.81		 0.98
0.4
0.89
12.6		 2.49
1.4
1.21
1.89		 0.8
0.49
0.78
0.44		 1.32
1.54
1.33
2.22		 1.89
1.01
1.67
1.57		 1.31
1.08
0.22
1.31		 2.12
1.77
0.09
1.09		 pmol/ug DNA
     (BCH081028) PA(36:4) -
-
-
0.74		 1.14
0.71
0.43
0.71		 1.37
0.86
0.71
0.84		 0.54
0.44
0.84
0.85		 0.77
0.85
0.88
0.61		 1.45
0.69
0.81
1.76		 1.09
1.36
1.43
3.81		 0.69
0.59
0.64
0.66		 pmol/ug DNA
     (BCH081209) PA(36:4) -
-
-
0.61		 1
1.05
1.04
0.91		 1.43
0.97
0.87
1.09		 1.36
0.85
1.09
1.1		 1.23
1.29
1.14
1.14		 1.37
1.2
1.12
1.18		 1.21
1.23
1.15
1.46		 1.08
1.33
1.55
1.24		 pmol/ug DNA
     (BCH081021) PA(38:1) -
-
-
-		 1.97
2.35
-
12.3		 2.04
1.09
2.15
1.65		 0.74
0.56
1.25
0.53		 1.02
1.37
2.2
0.81		 0.42
0.92
1.58
1.39		 0.94
1.46
3.8
1.36		 1.35
1.81
-
1.27		 pmol/ug DNA
     (BCH081028) PA(38:1) -
-
-
1.35		 2.81
1.59
1.45
2.23		 1.7
2.85
2.17
1.7		 2.66
2.17
1.56
1.84		 2.26
2.62
2.51
2.94		 4.59
2.47
3.14
3.41		 4.28
4.41
3.72
1.2		 2.6
1.73
0.86
3.77		 pmol/ug DNA
     (BCH081209) PA(38:1) -
-
-
0.23		 0.25
0.18
0.16
0.25		 0.2
0.28
0.15
0.31		 0.18
0.12
0.15
0.18		 0.08
0.53
0.17
0.19		 0.29
0.16
0.18
0.4		 0.11
0.26
0.15
0.63		 0.27
0.21
0.21
0.38		 pmol/ug DNA
     (BCH081021) PA(38:2) -
-
-
0.84		 1.82
-
-
-		 4.93
2.26
2.71
3.59		 1.25
0.58
0.82
0.84		 2.34
2.7
2.42
1.59		 1.29
1.42
3.47
2.97		 1.06
0.97
1.01
1.14		 1.28
1.71
0.55
1.32		 pmol/ug DNA
     (BCH081028) PA(38:2) -
-
-
0.18		 5.16
3.23
1.92
1.29		 1.95
0.43
0.99
1.46		 0.26
2.18
1.87
0.13		 0.64
2.57
0.27
2.88		 6.09
1.28
-
3.86		 0.67
2.54
2.53
1.6		 0.92
1.43
1.33
1.55		 pmol/ug DNA
     (BCH081209) PA(38:2) -
-
-
1.94		 0.62
0.8
1.15
0.67		 1.03
0.97
0.78
0.86		 0.76
0.73
1.19
0.77		 0.89
1.02
1.34
1.01		 1.02
0.81
0.7
1.01		 0.71
0.69
1.07
1.23		 1.19
1.46
1.25
0.69		 pmol/ug DNA
     (BCH081021) PA(38:3) -
-
-
2.76		 1.18
3.57
1.82
-		 2.06
1.37
2.72
-		 2.86
3.08
1.9
1.69		 1.56
3.17
3.23
2.65		 4.4
1.58
2.44
1.13		 4.2
4.04
4.33
4.4		 6.57
7.61
2.44
5.18		 pmol/ug DNA
     (BCH081028) PA(38:3) -
-
-
2.57		 5.3
3.41
2.11
3.26		 2.35
3.32
3.07
3.12		 2.67
2.41
2.46
2.49		 2.25
2.98
3.55
3.72		 -
3.36
3.46
-		 3.4
3.69
-
4.61		 2.41
3.06
2.26
2.19		 pmol/ug DNA
     (BCH081209) PA(38:3) -
-
-
4.27		 1.99
2.41
2.36
2.4		 2.62
2.49
2.19
2.46		 2.7
2.18
2.72
2.62		 2.64
1.44
2.64
2.13		 2.62
2.24
2.94
1.78		 1.63
2.92
2.79
3.9		 1.39
1.81
3.87
2.15		 pmol/ug DNA
     (BCH081021) PA(38:4) -
-
-
10.8		 11.2
3.63
11.1
35.4		 6.22
14.6
4.56
11.8		 3.23
2.61
10.7
11.4		 14.6
4.68
3.24
4.77		 4.24
12.8
9.16
10.7		 3.88
3.74
4.32
3.68		 5.81
5.82
2.23
4.75		 pmol/ug DNA
     (BCH081028) PA(38:4) -
-
-
6.09		 13.9
8.74
6.93
10.3		 7.45
8.53
9.19
9.34		 9.44
8.52
7.12
6.85		 8.89
9.93
9.71
11		 17.1
9.63
10.5
10.6		 10.5
13.1
13.5
11.9		 6.3
7
4.63
6.7		 pmol/ug DNA
     (BCH081209) PA(38:4) -
-
-
11.2		 3.78
4.3
4.37
5.01		 4.63
5.34
4.45
4.77		 4.77
4.29
4.39
4.47		 4.62
4.69
4.61
4.5		 5.1
4.26
4.84
4.97		 3.08
5.13
4.17
6.3		 4.78
5.45
6.31
4.94		 pmol/ug DNA